Immuno-oncology and immunotherapy Part A

Lorenzo Galluzzi is Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College, Honorary Assistant Professor Adjunct with the Department of Dermatology of the Yale School of Medicine, Honorary Associate Professor with the Faculty of Medicine of the University of Paris, and Faculty Member with the Graduate School of Biomedical Sciences and Biotechnology of the University of Ferrara, the Graduate School of Pharmacological Sciences of the University of Padova, and the Graduate School of Network Oncology and Precision Medicine of the University of Rome “La Sapienza”. Moreover, he is Associate Director of the European Academy for Tumor Immunology and Founding Member of the European Research Institute for Integrated Cellular Pathology. Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. He has published over 450 articles in international peer-reviewed journals and is the Editor-in-Chief of four journals: OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, Methods in Cell biology, and Molecular and Cellular Oncology (which he co-founded in 2013). Additionally, he serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease, Pharmacological Research and iScience.

1. Preface
Charpentier Maud and Norma Bloy
2. Tumor slice culture system for ex vivo immunotherapy studies
Fernando Aranda
3. Degranulation assay to evaluate NK cell natural and antibody-dependent cell-mediated cytotoxicity against A549 tumor spheroids
Francisco Borrego
4. ELISpot assay to quantify peptide-specific IFNγ production by splenocytes in a mouse tumor model of radiation therapy
Jihane Boustani
5. Flow cytometry detection and quantification of circulating leucocyte subpopulations in mice after brain irradiation
Julie Coupey, Marine M. Leblond, Erika S. Hue and Samuel Valable
6. Assessment of pDC functional capacity upon exposure to tumor-derived soluble factors
Anna Fialová
7. Flow cytometry-assisted analysis of phenotypic maturation markers on an immortalized dendritic cell line
Lorenzo Galluzzi
8. Cytofluorometric assessment of calreticulin exposure on CD38+ plasma cells from the human bone marrow
Lorenzo Galluzzi
9. Generation of transmitochondrial cybrids in cancer cells
Lorenzo Galluzzi
10. Identification and analysis of alloreactive T lymphocytes from peripheral blood mononuclear cells
Enrico Lugli, Alberto Susana, Giovanni Galletti, Gabriele De Simone and Chiara Camisaschi
11. Optimization and validation of a multiparametric flow cytometric assay for monitoring peripheral blood TREGs
Iole Macchia Sr., Floriana Iacobone and Francesca Urbani
12. Characterization of NK cells infiltrating irradiated murine tumors through flow cytometry
Céline Mirjolet, Jérémy Baude, Riad Ladjohounlou, Emeric Limagne, Lisa Froidurot and Véronique Morgand
13. Assessment of translation rate in leukemic cells and immune cells of the tumor microenvironment by OPP protein synthesis assay
Jerome Paggetti, Vanessa Klapp, Anne Largeot, Ozgu Gumustekin and Etienne Moussay