Schmidtea Mediterranea
Springer-Verlag New York Inc.
978-1-0716-3277-2 (ISBN)
Authoritative and practical, Schmidtea mediterranea: Methods and Protocols provides both experts in the field and newcomers with the best possible toolbox for their everyday lab work utilizing this valuable model.
Building Phylogenies from Transcriptomic Data.- Isolation and Library Preparation of Planarian piRNAs.- Genome-Wide Analysis of Planarian piRNAs.- Combining Fluorescent In Situ Hybridization with Immunofluorescence and Lectin Staining in Planarians.- Colorimetric Whole-Mount In Situ Hybridization in Planarians.- Single Molecule Fluorescent In Situ Hybridization (smFISH) on Whole-Mount Planarians.- Whole-Mount In Situ Hybridization in Large Sexual Schmidtea mediterranea.- Preparing Planarian Cells for High-Content Fluorescence Microscopy Using RNA In Situ Hybridization and Immunocytochemistry.- An RNA/DNA-Based Flow Cytometry Approach for Isolating Slow-Cycling Stem Cells.- ACME Dissociation-Fixation, Flow Cytometry, and Cell Sorting of Freshwater Planarian Cells.- Papain-Based Dissociation of Schmidtea mediterranea Cells.- Live Immunostaining and Flow Cytometry of Schmidtea mediterranea Cells.- Live Imaging in Planarians: Immobilization and Real-Time Visualization of Reactive Oxygen Species.- A Planarian Model System to Study Host-Pathogen Interactions.- TUNEL Staining in Sections of Paraffin-Enabled Planarians.- Quantitative Analysis of Planarian Pigmentation.- mRNA Transfection of S. mediterranea Cells for Luminescence Analysis.
Erscheinungsdatum | 13.07.2024 |
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Reihe/Serie | Methods in Molecular Biology |
Zusatzinfo | 50 Illustrations, color; 4 Illustrations, black and white; XI, 280 p. 54 illus., 50 illus. in color. |
Verlagsort | New York, NY |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Zellbiologie |
Schlagworte | Genomics and transcriptomics • immunohistochemistry • In situ Hybridization • Large dataset analysis • Planarians • Stem Cell Biology |
ISBN-10 | 1-0716-3277-9 / 1071632779 |
ISBN-13 | 978-1-0716-3277-2 / 9781071632772 |
Zustand | Neuware |
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