Phagocytosis and Phagosomes

Bacterial Binding, Phagocytosis, and Killing: Measurements Using Colony Forming Units.- Analysis of Human and Mouse Neutrophil Phagocytosis by Flow Cytometry.- Quantifying Phagocytosis by Immunofluorescence and Microscopy.- Methods for Quantitative Efferocytosis Assays.- Analysis of Efferocytic Receptor Dynamics and Synapse Formation in a Frustrated Efferocytosis Model.- Super-Resolution Spinning-Disk Confocal Microscopy Using Optical Photon Reassignment (SoRa) to Visualize the Actin Cytoskeleton in Macrophages.- Filamentous Bacteria as Targets to Study Phagocytosis.- The Derivation and Use of HoxB8-Driven Conditionally Immortalized Macrophages.- Quantitative Immunofluorescence to Study Phagosome Maturation and Resolution.- Approaches to Measuring Reductive and Oxidative Events in Phagosomes.- Measuring Phagosomal pH by Fluorescence Microscopy.- Multiplexed Phagosomal Assays for the Detection and Quantification of Bidirectional Exchange Between the Phagolysosomal Lumen and Extracellular Space.- Quantitative Spatio-Temporal Analysis of Phagosome Maturation in Live Cells.- Measurement of Salmonella enterica Internalization and Vacuole Lysis in Epithelial Cells.- Microscopy-Based Tracking and Quantification Methods to Study Phagosome Resolution.- Isolation of Polystyrene Bead-Induced Phagosomes for Western Blotting.- Biochemically Reconstituted Fusion of Phagosomes with Endosomes and Lysosomes.- An In Vitro System to Analyze Generation and Degradation of Phagosomal Phosphatidylinositol Phosphates.- Examining Phagosomal Pattern Recognition Receptor-Dependent Signaling and Antigen MHC-II Presentation from Phagosomes in Dendritic Cells.- Examining the Kinetics of Phagocytosis-Coupled Inflammasome Activation in Murine Bone Marrow-Derived Dendritic Cells.- Analysis of LC3-Associated Phagocytosis and Antigen Presentation in Macrophages and B Cells.- Visualizing Phagocytic Cargo In Vivo from Engulfment to Resolution in Caenorhabditis elegans.- Assessing the Phagosome Proteome by Quantitative Mass Spectrometry.- Using Ion Substitution and Fluid Indicators to Monitor Macropinosome Dynamics in Live Cells.