Spermatogonial Stem Cells
Springer-Verlag New York Inc.
978-1-0716-3141-6 (ISBN)
Cutting-edge and comprehensive, Spermatogonial Stem Cells: Methods and Protocols is a valuable resource for both new and experiences investigators looking to expand their knowledge in this developing field.
Introduction: The Why’s and How’s for Studying Spermatogenesis and Spermatogonial Stem Cells.- Optimizing Testicular Fixation-Embedding Procedures for an Improved Morphofunctional Evaluation of Mammalian Spermatogonia.- Single-Molecule Fluorescence In Situ Hybridization for Spatial Detection of mRNAs in Sections of Mammalian Testes.- Bulk and Single-Cell RNA-Seq Analyses for Studies of Spermatogonia.- Profiling the Epigenetic Landscape of the Spermatogonial Stem Cell – Part 1: Epigenomics Assays.- Profiling the Epigenetic Landscape of the Spermatogonial Stem Cell – Part 2: Computational Analysis of Epigenomics Data.- Primary Cultures of Spermatogonia and Testis Cells.- Reconstitution of Human Prospermatogonial Development from Human Induced Pluripotent Stem Cells.- RNA Interference as a Method of Gene Knockdown in Cultured Spermatogonia.- Isolation of Undifferentiated Spermatogonia from Adult and Developing Mouse Testes.- Spermatogonial Transplantation.- Viral Transduction of Mammalian Spermatogonial Stem Cells.- Pharmacological Treatment of Neonatal and Juvenile Mice to Study Spermatogenesis.- Models and Methods for Evaluating Regeneration of Spermatogenesis After Cytotoxic Treatments.- Defining Gene Function in Spermatogonial Stem Cells through Conditional Knockout Approaches.- Lineage Tracing of Spermatogonial Stem Cells within the Male Germline.- Perspectives: Approaches for Studying Livestock Spermatogonia.- Perspectives: Methods for Evaluating Primate Spermatogonial Stem Cells.
Erscheinungsdatum | 01.06.2024 |
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Reihe/Serie | Methods in Molecular Biology |
Zusatzinfo | 52 Illustrations, color; 8 Illustrations, black and white; X, 370 p. 60 illus., 52 illus. in color. |
Verlagsort | New York, NY |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie |
Schlagworte | Conditional Knockout • Cytotoxic Regeneration • Immunostaining • mammalian gem cell biology • RNAi Knockdown |
ISBN-10 | 1-0716-3141-1 / 1071631411 |
ISBN-13 | 978-1-0716-3141-6 / 9781071631416 |
Zustand | Neuware |
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