Transmembrane Signaling Protocols

Overviews.- Transmembrane Signaling by G Protein-Coupled Receptors.- Crosstalk Coregulation Mechanisms of G Protein- Coupled Receptors and Receptor Tyrosine Kinases.- Protein-Protein Interactions in Signaling Cascades.- Specific Topics.- Biological Role of the CXCR4-SDF-1 Axis in Normal Human Hematopoietic Cells.- Functional Expression of CXCR4 in Saccharomyces cerevisiae in the Development of Powerful Tools for the Pharmacological Characterization of CXCR4.- Characterization of Constitutively Active Mutants of G Protein-Coupled Receptors.- G Protein-Coupled Receptor Dimerization and Signaling.- Real-Time Analysis of G Protein-Coupled Receptor Signaling in Live Cells.- Isolation of Membrane Rafts and Signaling Complexes.- Methods for the Study of Signaling Molecules in Membrane Lipid Rafts and Caveolae.- Bioluminescence Resonance Energy Transfer to Monitor Protein-Protein Interactions.- Identification of Interacting Proteins Using the Yeast Two-Hybrid Screen.- Analysis of PDZ Domain Interactions Using Yeast Two-Hybrid and Coimmunoprecipitation Assays.- Clustering Assay for Studying the Interaction of Membrane Proteins with PDZ Domain Proteins.- Mammalian Cell Microinjection Assay to Study the Function of Rho Family Guanosine Triphosphatases.- Affinity-Based Assay of Rho Guanosine Triphosphatase Activation.- Assay of Phospholipase D Activity in Cell-Free Systems.- Reconstitution System Based on Cytosol-Depleted Cells to Study the Regulation of Phospholipase D.- Analysis of Global Gene Expression Profiles Activated by Chemoattractant Receptors.- Genetic Reconstitution of Bone Marrow for the Study of Signal Transduction Ex Vivo.- Proteomic Analysis of Human Neutrophils.