The Immunological Synapse Part C

Clément Thomas, Ph.D. is a research group leader at the Department of Cancer Research of the Luxembourg Institute of Health, Luxembourg. He received his Ph.D. in Molecular and Cellular Biology from the University of Strasbourg. Over the last 15 years, his group has developed extensive knowledge and expertise in the mechanisms regulating actin cytoskeleton organization and dynamics, as well as in live cell imaging approaches. His current research is centered on the role of the actin cytoskeleton in tumor immune evasion. Lorenzo Galluzzi is Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College, Honorary Assistant Professor Adjunct with the Department of Dermatology of the Yale School of Medicine, Honorary Associate Professor with the Faculty of Medicine of the University of Paris, and Faculty Member with the Graduate School of Biomedical Sciences and Biotechnology of the University of Ferrara, the Graduate School of Pharmacological Sciences of the University of Padova, and the Graduate School of Network Oncology and Precision Medicine of the University of Rome “La Sapienza”. Moreover, he is Associate Director of the European Academy for Tumor Immunology and Founding Member of the European Research Institute for Integrated Cellular Pathology. Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. He has published over 450 articles in international peer-reviewed journals and is the Editor-in-Chief of four journals: OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, Methods in Cell biology, and Molecular and Cellular Oncology (which he co-founded in 2013). Additionally, he serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease, Pharmacological Research and iScience.

Preface
Clément Thomas and Lorenzo Galluzzi
1. Analysis of immune synapse by STED imaging and 3D quantitative colocalization of lytic granule markers
Claudio Giraudo
2. Imaging the immune synapse: three-dimensional analysis of the immune synapse
Manuel Izquierdo
3. Image processing approaches for microtubule remodeling quantification at the immunological synapse
Andres Alcover, Marie Juzans, Celine Cuche and Vincenzo Di-Bartolo
4. Isolation and characterization of primary NK cells and their KIR2DL1+ population enrichment
Mira Barda-Saad
5. Chapter title to be confirmed
Johannes B. Huppa and Janett Göhring
6. Flow Cytometry Conjugate Formation Assay between Natural Killer (NK) Cells and Their Target Cells
Jacques Zimmer
7. Quantifying force-mediated antigen extraction in the B cell immune synapse using DNA-based tension sensors
Katelyn Spillane, Anna Bajur and Hannah McArthur
8. Chapter title to be confirmed
Rose Thierry
9. Quantitative analysis of the B Cell immune synapse formation using imaging techniques
Isabel Yuseff
10. chapter title yet to confirmed
Rose Thierry
11. Expansion of ?d T cells and their use in in vitro cytotoxicity assays
Maria Alejandra Parigiani
12. A Comprehensive Guide to Studying the Immunological Synapse Using Imaging Flow Cytometry
Céline Hoffmann, Clément Thomas, Liza Filali, Andrea Michela Biolato and Max Krecke