Carbohydrate-Protein Interactions

A low-volume, parallel copper-bicinchoninic acid (BCA) assay for glycoside hydrolases.- Measuring enzyme kinetics of glycoside hydrolases using the 3,5-dinitrosalicylic acid assay.- Analyzing activities of lytic polysaccharide monooxygenases by liquid chromatography and mass spectrometry.- Carbohydrate depolymerization by intricate cellulosomal systems.- Biomechanical weakening of paper and plant cell walls by bacterial expansins.- Affinity Electrophoresis for Analysis of Catalytic Module-Carbohydrate Interactions.- Quantifying CBM carbohydrate interactions using microscale thermophoresis.- Qualitative and quantitative characterization of protein-carbohydrate interactions by NMR spectroscopy.- Isothermal Titration Calorimetry for quantification of protein-carbohydrate interactions.- Surface plasmon resonance analysis for quantifying protein-carbohydrate interactions.- Bioinspired polymer assemblies of plant cell walls for measuring protein-carbohydrate interactions by FRAP.- CBMs as probes to explore plant cell wall heterogeneity using immunocytochemistry.- Flow Cytometry-based Detection of Siglec Ligands.-Extraction and Verification of Mouse and Human Mucins from Tissue and Fecal Material.- Analysis of complex carbohydrate composition in plant cell wall using Fourier transformed mid-infrared spectroscopy.- Separation and Visualisation of Glycans by Fluorophore-Assisted Carbohydrate Electrophoresis.- A rapid protocol for preparing 8-aminopyrene-1,3,6-trisulfonate-labelled glycans for capillary electrophoresis-based enzyme assays.- Visualization of Carbohydrate Uptake using Fluorescent Polysaccharides.- Long-read Metagenomics and CAZyme discovery.- Identification of genes involved in the degradation of lignocellulose using comparative transcriptomics.- Isolation and preparation of extracellular proteins from lignocellulose degrading fungi for comparative proteomic studies using mass spectrometry.