Advanced Methods in Structural Biology

Cloning and Recombinant Protein Expression in Lactococcus lactis.- E6 Tagged Protein Production, Extraction, and Purification from Escherichia coli Lysate.- Mini-Bioreactor Platform for Membrane Proteins Production in Komagataella pastoris.- Recombinant Protein Production Using the Baculovirus Expression Vector System (BEVS).- High-Throughput Production and Optimization of Membrane Proteins after Expression in Mammalian Cells.- Fluorescent Fusion Proteins Expression in Plant Cells.- Functional Expression of the Ectodomain of Plant Receptor Kinases in Plant Suspension Culture.- A Simple Purification Method for Heat Stable Recombinant Low Molecular Weight Proteins and Peptides Via GST-Fusion Products.- eGFP as an All-in-One Tag for Purification of Membrane Proteins.- Effects of Ionic Liquids as Additives on Protein Crystallization.- Screening of Buffers and Additives for Protein Stabilization by Thermal Shift Assay: A Practical Approach.- Biophysical Characterization of Membrane Proteins.- Expression in Saccharomyces cerevisiae and Purification of a Human Phospholipid Flippase.- Preparation of Protein-Enriched Outer Membrane Vesicles from Escherichia Coli for In Situ Structural Biology of Outer Membrane Proteins.- Receptor-Based Virtual Screening of Large Libraries in a Multi-Level in Silico Approach.- Protein-Protein Interaction Studies Using Molecular Dynamics Simulation.- Isolation of Bacterial Extracellular Vesicles and Identification of their Protein Cargo.- Study of Protein Conformational Dynamics Using Hydrogen/Deuterium Exchange Mass Spectrometry.- Protein Interaction Analysis by Surface Plasmon Resonance.- High-Throughput Protein Crystallization in an Integrated Droplet-Based Microfluidic Platform.- Sample Preparation for Time-Resolved Serial Crystallography: Practical Considerations.- Using Small-Angle X-Ray Scattering to Characterize Biological Systems:A General Overview and Practical Tips.- Nuclear Magnetic Resonance-Guided Structural Analysis of Moderate-Affinity Protein Complexes with Intrinsically Disordered Polypeptides.- Protein Structural Analysis by Cryogenic Electron Microscopy.