Cryo-Electron Microscopy in Structural Biology

SECTION I: Cryo-EM in Cell Biology - Structure Determination of Cellular Organelle
1. Atomic-resolution protein structure determination by cryo-EM
2. Sub-ångström cryo-EM Structure of a prion proto-fibrils
3. Cryo-EM structure of native human uromodulin, a zona pellucida module polymer
4. Chromatosome, telomere structure and genome maintenance using cryo-EM
5. Cryo-EM of the dynamin super-family proteins
6. Mito-ribosome structure using cryo-EM
SECTION II: Technology Platform & Evolving Technologies
1. Cryo-EM data in the cloud computing
2. The silent revolution of cryo-EM and GPCR structures
3. Development of world-first multi-gas plasma focused ion beam microscope
4. 3-D printed cell culture grid holders for improved cellular specimen preparation in cryo- electron microscopy
5. Local resolution estimates of cryo-EM reconstruction
6. A new cryo-EM system for electron 3D crystallography
SECTION III: Cryo-EM in Virology - High-Resolution Structures of Viruses
1. Cryo-Electron Microscopy structure of adeno-associated virus-DJ bound by a heparinoid pentasaccharide
2. Cryo-EM and tomography of vesicular stomatitis viruses
3. High-resolution structures of HIV-1 gag cleavage mutants
4. Cryo-electron microscopy of the T7 bacteriophage portal and fiber-less tail complex
5. SARS-Co-2 spike protein structure determination
SECTION IV: Cryo EM in Membrane Biology – Probing the structures of Membrane Proteins
1. Structure and activity of lipid bilayer within a membrane-protein transporter
2. Cryo-EM structure of human Rhodopsin bound to an inhibitory G protein
3. Cryo-EM structure of the acetylcholine receptor that cause congenital myasthenic syndromes
4. Structure of the mitochondrial F-ATPase as determined by electron cryo-microscopy
5. Cryo-EM structure of cardiac amyloid fibrils from an immunoglobulin light chain AL amyloidosis patient
SECTION V: Cryo-EM in Molecular Biology - Structural Insights of Nuclear and Gene Machineries
1. Structural information from cryo-EM studies on multicomponent molecular assemblies such as those found in DNA-double-strand-break repair
2. Cryo-EM structure of the DNA-PK holoenzyme
3. Cryo-EM reconstruction at 4.0 Å of human Polymerase III
4. Cryo-electron microscopy structures of UV-DDB bound to nucleosome
5. Cryo-electron microscopy of the chromatin fiber
6. Time-resolved cryo-EM studies in the ribosome dynamics
SECTION VI: Cryo-Tomography and Emerging Technologies
1. Cryo-Electron Tomography in membrane protein structural research
2. Cryo-tomography of membrane transporters involved in neurological psychiatric disease
3. Enveloped viruses and coated vesicles - electron cryo-microscopy and tomography
4. Electron cryo-tomography in the study of autophagy or bacterial secretion systems
5. DNA Origami sign posts for identifying proteins on cell membranes by cryo-tomography
6. 3-D deconvolution processing for STEM cryo-tomography
SECTION VII: Applications of Cryo-EM/ET in Drug Discovery
1. Cryo-EM to enable research and drug discovery of CD20 in complex with the therapeutic monoclonal antibody rituximab
2. Fragment-based drug discovery using cryo-EM
3. CryoEM approaches to study allosteric regulation of GTP cyclohydrolase I
4. Cryo-EM in drug discovery
5. Structural insights of an advanced vaccine candidate as derived from SARS-CoV-2 spike protein
6. Unleashing the power of Cryo-EM
Epilogue