Bacterial Chromatin

Shedding light on bacterial chromosome structure: exploring the significance of 3C-based approaches.- Circuit topology analysis of single cell Hi-C data.- GeF-seq: a simple procedure for base pair resolution ChIP-seq.- ChIP-qPCR of FLAG-tagged proteins in bacteria.- Genomic SELEX screening of regulatory targets of transcription factors.- High-resolution characterization of DNA/protein complexes in living bacteria.- High throughput mapping of bacterial chromosomal conformations using massively multiplexed Mu transposition.- In vivo genomic supercoiling mapping using Psora-seq.- Modular assembly of synthetic secondary chromosomes.- Visualization, quantification, and statistical evaluation of dynamics for DNA-binding proteins in bacteria by Single molecule tracking.- Genetic approaches to study the interplay between transcription and nucleoid-associated proteins in Escherichia coli.- Genetic engineering of Bacillus subtilis using competence-induced homologous recombination techniques.- Atomic force microscopy imaging and analysis of prokaryotic genome organization.- Atomic force microscopy characterisation of reconstituted protein-DNA complexes.- Approaches for determining DNA persistence length using Atomic Force Microscopy.- Dynamic light scattering of DNA-ligand complexes.- Microscale thermophoresis analysis of chromatin interactions.- In vitro transcription assay to quantify effects of H-NS filaments on RNA chain elongation by RNA polymerase.- Methods to quantitatively measure topological changes induced by DNA binding proteins in vivo and in vitro.- Quantitative determination of DNA bridging efficiency of chromatin proteins.- Measuring Partition Coefficients of in vitro Biomolecular Condensates using Fluorescence Correlation Spectroscopy.- Tethered Particle Motion analysis of the DNA binding properties of architectural proteins.- Quantitation of DNA binding affinity using Tethered Particle Motion.- Unravelling DNA organization with single-molecule Force Spectroscopy using Magnetic Tweezers.- Approaches to the full and partial chemical synthesis of proteins.- Deciphering Sequence-Specific DNA Binding by H-NS using Molecular Simulation.- Molecular dynamics simulations of a feather-boa model of a bacterial chromosome.- Replicating Chromosomes in Whole-Cell Models of Bacteria.