Bioinorganic Chemistry

Rosette M. Roat-Malone, PhD, is Adjunct Professor of Chemistry at Washington College in Chestertown, Maryland. She developed the advanced bioinorganic chemistry course that formed the basis for this book's predecessor, Bioinorganic Chemistry: A Short Course. Her research in the reactions of platinum coordination compounds used as anticancer agents with biological molecules has been supported by the National Science Foundation, the Petroleum Research Fund, and the Research Corporation.

Chapter 1: Inorganic Chemistry Essentials. Introduction . Essential Chemical Elements. Metals in Biological Systems: A Survey. Inorganic Chemistry Basics. Biological Metal Ion Complexation. Thermodynamics. Kinetics. Electronic and Geometric Structures of Metals in Biological Systems. Bioorganometallic Chemistry. Electron Transfer. Introduction to Biological Metal Homeostasis, Transport, and Storage. Chapter 2: Biochemistry Fundamentals. Introduction. Proteins. Amino Acid Building Blocks. Protein Structure. Protein Sequencing and Proteomics. Protein Function, Enzymes and Enzyme Kinetics. Nucleic Acids. DNA and RNA Building Blocks. DNA and RNA Molecular Structures. Transmission of Genetic Information. Genetic Mutations and Site-Directed Mutagenesis. Genes and Cloning. Genomics and the Human Genome. The ATP-binding cassette (ABC) Transporter Superfamily. Chapter 3: Instrumental Methods. Introduction. Analytical Instrument-Based Methods. Spectroscopy. X-ray Absorption Spectroscopy (XAS) and Extended X-ray Absorption Fine Structure (EXAFS). Theoretical Aspects and Hardware. Descriptive Examples. X-ray Crystallography. Introduction. Crystallization and Crystal Habits. Theory and Hardware. Descriptive Examples. Electron Paramagnetic Resonance. Theory and Determination of g-values. Hyperfine and Superhyperfine Interactions. Descriptive Examples. Nuclear Magnetic Resonance. Theoretical Aspects. Nuclear Screening and the Chemical Shift. Spin-Spin coupling. Techniques of Spectral Integration and Spin-Spin Decoupling. Nuclear Magnetic Relaxation. The nuclear Overhauser Effect (NOE). Obtaining the NMR spectrum. Two-dimensional NMR Spectroscopy. Correlation Spectroscopy (COSY). Nuclear Overhauser Effect Spectroscopy (NOESY). Descriptive Examples. Mossbauer Spectroscopy. Theoretical Aspects. Quadrupole Splitting and the Isomer Shift. Magnetic Hyperfine Interactions. Descriptive Examples. Atomic Force Microscopy. Fast Methods. Stopped-Flow Kinetic Methods. Flash Photolysis. Time-Resolved Crystallography. Chapter 4: Introduction to Computer-Based Methods. Computer Hardware . Molecular Modeling and Molecular Mechanics. Introduction to MM. Molecular Modeling, Molecular Mechanics and Molecular Dynamics. Biomolecule Modeling. Molecular Modeling Descriptive Examples. Quantum Mechanics-Based Computational Methods. Introduction. Ab-initio Methods. Density Functional Theory. Semi-empirical Methods. Computer Software for Chemistry. Mathematical Software. World Wide Web Online Resources. Nomenclature and Visualization Resources. Online Societies, Literature, Materials, Equipment Webservers. Chapter 5: Group I and II metals in biological systems. Transport and storage of metal ions. Homeostasis involving Na+, K+, Ca2+, Mg2+, Zn2+ (as well as P, Cl, and H). Movement of molecules and ions across membranes. Proton pumps. Ion pumps. Active versus passive transport . Na+/ K+ ATPases. Magnesium dependent biomolecules. Kinases. ATP-pumps. Catalytic RNA and Magnesium. Magnesium in chlorophyll. Calcium dependent biomolecules. Calmodulins and triggering. Biochemistry of nitrogen oxides, nitroxyl, and peroxynitrite ion. Chapter 6: Iron Containing Proteins and Enzymes. Iron-containing proteins with porphyrin ligand systems. Hemoglobin and Myoglobin (first edition material somewhat condensed). Cytochrome P-450: a monooxygenase. Iron-containing cytochromes in photosynthesis. Green plant cytochromes. Cytochrome bc559. Cytochrome bf. Bacterial cytochromes. Cytochrome c2. Cytochrome bc1. Iron-containing cytochromes in the respiratory pathway. Cytochrome c. Cytochrome c oxidase. Non-Heme Iron-containing proteins. MMO-Methane Monooxygenase. Catechol dioxygenases. Iron-oxo clusters. Transferrin for metal transport . Ferritin for metal storage. Hemerythrin. Purple acid phosphatase. Iron-sulfur cluster proteins. Rubredoxin. Ferrodoxins. Aconitase. Chapter 7: Enzymes containing zinc, nickel, and enzymes with multiple metal cofactors. Zinc homeostasis, transport, and storage. Zinc Enzymes. Alkaline phosphatase. Alcohol dehydrogenase. Aspartate transcarbamoylase. Carbonic anhydrase. Carboxypeptidase A and B. Zinc Fingers. Nickel Enzymes. Urease. F430. Enzymes with multiple metal cofactors. Hydrogenases. Carbon monoxide dehydrogenases. Chapter 8 Disease, Disease Diagnosis and Treatment Utilizing Inorganic Species. Metal Transport and Metallochaperones. Disease States. Metal deficiency. Metal toxicity. Copper toxicity. FALS. Wilson and Menkes diseases. Disease Diagnosis. Technetium Imaging Agents. Gadolinium MRI Imaging Agents. Quantum Dots for In vivo Imaging. Metals in Medicine. Manganese superoxide mimetics. Vanadium-based Diabetes Drugs. Platinum-containing Anticancer Agents. Nanomedicine.