Recombinant Protein Expression in Mammalian Cells

PEI-Mediated Transient Gene Expression in CHO Cells.- Transient Co-Expression of Membrane Protein Complexes in Mammalian Cells.- The Use of Baculovirus-Mediated Gene Expression in Mammalian Cells for Recombinant Protein Production.- Production and Purification of Adeno-Associated Viral Vectors (AAV) Using Orbitally Shaken HEK293 Cells.- Protein Expression via Transient Transfection of Mammalian Cells in a WAVE Bioreactor.- Small-Scale Cultivation and Transfection of ExpiCHO and HEK293E Cells in Single-Use Orbitally Shaken Bioreactors.- Recombinant Protein Production from Stable CHO Cell Pools.- A Versatile Method for Inducible Protein Production in 293 Cells via the PiggyBac Transposon System.- Recombinant CHO Cell Pool Generation Using PiggyBac Transposon System.- Stable Expression by Lentiviral Transduction of Cells.- Expression of Multispecific Antibodies.- Expression of Bispecific Antibodies Using Cellular Display.- Generation of Monoclonal Chinese hamster Ovary Cell Lines Using DISPENCELL-S3.- Enhancing Cell Line Stability by CRISPR/Cas9-Mediated Site-Specific Integration Based on Histone Modifications.- Bench-Scale Stirred-Tank Bioreactor for Recombinant Protein Production in Chinese Hamster Ovary (CHO) Cells in Suspension.- Application of the CRISPR/Cas9 Gene Editing Method for Modulating Antibody Fucosylation in CHO Cells.- Development of Responsive Promoters and Their Utilization for Stable CHO Sensor Cell Lines.- CRISPR-Deletion of miR-27 Impacts Recombinant Protein Production in CHO Cells.- High-Throughput Protein Expression Screening of Cell Surface Protein Ectodomains.- Genome Wide High Throughput RNAi Screening for Identification of Genes Involved in Protein Production.- Purification and Quality Control of Recombinant Proteins Expressed in Mammalian Cells: A Practical Review.