Axon Regeneration

Defining selective neuronal resilience and identifying targets for neuroprotection and axon regeneration using single-cell RNA sequencing: Experimental approaches.- Defining selective neuronal resilience and identifying targets of neuroprotection and axon regeneration using single-cell RNA sequencing – computational approaches.- Retinal Ganglion Cell Axon Fractionation.- Analysis of Immediate Early Gene Expression Levels to Interrogate Changes In Cortical Neuronal Activity Patterns Upon Vision Loss.- Non-coding regulatory RNAs: Isolation and analysis of neuronal circular RNAs and microRNAs.- Transneuronal delivery of cytokines to stimulate mammalian spinal cord regeneration.- Epigenomic Profiling of Dorsal Root Ganglia Upon Regenerative and Non-Regenerative Axonal Injury.- Profiling locally translated mRNAs in regenerating axons.- Analysis of axonal regrowth and dendritic remodeling after optic nerve crush in adult zebrafish.- Assaying OpticNerve Regeneration in Larval Zebrafish.- Surgical Methods in Postmetamorphic Xenopus laevis: Optic Nerve Crush Injury Model.- Generating widespread and scalable retinal lesions in adult zebrafish by intraocular injection of ouabain.- A reproducible spinal cord crush injury in the regeneration permissive axolotl.- Live imaging of axonal dynamics after laser axotomy of peripheral neurons in zebrafish.- Rapid testing of gene function in axonal regeneration after spinal cord injury using larval zebrafish.- Translational Ribosomal Affinity Purification (TRAP) and Bionformatic RNA-Seq Analysis in Postmetamorphic  Xenopus laevis.- LCM-seq for Retinal Cell Layer-Specific Responses During Optic Nerve Regeneration.- Temporal-Based Dynamic Profiling of DNA Accessibility With ATAC-Seq in the Regenerating Retina of Zebrafish.- Quantitative Proteomics of Nervous System Regeneration: From Sample Preparation to Functional Data Analyses.- Live Cell Imaging of Dynamic Processes in Adult Zebrafish Retinal Cross-section Cultures.- PCNA staining of retinal cryosections to assess microglial/macrophage proliferation.- Using a Drosophila injury model to investigate RNA repair and splicing in axon regeneration.- Assessing rewiring of the retinal circuitry after inner retinal lesion zebrafish.- Analysis of visual recovery after optic nerve crush in adult zebrafish.