DNA Nanotechnology for Cell Research (eBook)

Preface

PART I. DNA Nanotechnology for Cellular Recognition (Cell SELEX, cell surface engineering)

1. DEVELOPING DNA APTAMER TOOLBOX FOR CELL RESEARCH
1.1 Cells and their complexity
1.2 DNA aptamers' characteristics and advantages
1.3 On-demand synthesis and screening of DNA aptamers
1.4 Toward a toolbox of DNA aptamers for cellular applications
1.5 Summary and Outlook

2. BACTERIAL DETECTION WITH FUNCTIONAL NUCLEIC ACIDS: ESCHERICHIA COLI AS A CASE STUDY
2.1 General Introduction on Bacteria
2.2 E. coli
2.3 Conventional Methods for General E. coli Detection
2.4 Biosensors for E. coli Detection
2.5 Conclusion

3. FROM LIGAND-BINDING APTAMERS TO MOLECULAR SWITCHES
3.1 Aptamers can be generated by SELEX
3.2 Various subtypes of SELEX have been invented
3.3 Riboswitches are natural RNA aptamers carrying expression platforms
3.4 Riboswitches use various mechanisms to regulate gene expression
3.5 Riboswitches are potential drug targets
3.6 Fusing aptamer with expression platform to construct artificial RNA switches
3.7 Conclusions

4. DNA NANOTECHNOLOGY-BASED MICROFLUIDICS FOR LIQUID BIOPSY
4.1 Introduction
4.2 DNA nanotechnology-based microfluidics for isolation of circulating targets
4.3 DNA nanotechnology-based microfluidics for release and detection of circulating targets
4.4 DNA-assisted microfluidics for single cell/vesicle analysis
4.5 Summary and Outlook

5. SPATIOTEMPORAL CONTROLLED CELL MEMBRANE ENGINEERING USING DNA NANOTECHNOLOGY
5.1 Background
5.2 DNA Modifications on the External Cell Membrane Surface
5.4 Perspectives

PART II. DNA Nanotechnology for Cell Imaging and Intracellular Sensing

6. METAL-DEPENDENT DNAZYMES FOR CELL SURFACE ENGINEERING AND INTRACELLULAR BIOIMAGING
6.1 Cellular Surface Engineering and Intracellular Bioimaging Show Great Potential in Biological and Medical Research
6.2 Metal-Specific DNAzymes: a Suitable Choice for Artificial Manipulation of Living Cells
6.3 Cell Surface Engineering by Programmable DNAzymes
6.4 Intracellular Imaging of Metal Ions with DNAzyme-Based Biosensors
6.5 Conclusion

7. DNA NANOMOTORS FOR BIOIMAGING IN LIVING CELLS

8. ILLUMINATING RNA IN LIVE CELLS WITH INORGANIC NANOPARTICLES-BASED DNA SENSOR TECHNOLOGY
8.1 RNA Detection and Imaging
8.2 RNA Imaging Based on Direct Hybridization
8.3 RNA Imaging Based on Strand Displacement Reactions
8.4 Signal-amplified RNA Imaging
8.5 Spatiotemporally Controlled RNA Imaging in Live Cells
8.6 Conclusion

9. BUILDING DNA COMPUTING SYSTEM FOR SMART BIOSENSING AND CLINICAL DIAGNOSIS
9.1 DNA computing
9.2 DNA-based computing devices for biosensing
9.3 DNA computing for clinical diagnosis
9.4 Conclusion

10. INTELLIGENT SENSE-ON-DEMAND DNA CIRCUITS FOR AMPLIFIED BIOIMAGING IN LIVING CELLS
10.1 DNA Circuit: The Promising Technique for Bioimaging
10.2 Non-enzymatic DNA Circuits
10.3 Intelligent Integrated DNA Circuits for Amplified Bioimaging
10.4 Stimuli-responsive DNA Circuits for Reliable Bioimaging
10.5 Conclusion and Perspectives

11. DNA NANOSCAFFOLDS FOR BIOMACROMOLECULES ORGANIZATION AND BIOIMAGING APPLICATIONS
11.1 Introduction
11.2 Assembly of DNA-scaffolded biomacromolecules
11.3 Application of DNA nanoscaffold for regulation of enzyme cascade reaction
11.4 DNA nanostructures empowered bioimaging technologies
11.5 Summary and outlook

PART III. DNA Nanotechnology for Regulation of Cellular Functions

12. ADOPTING NUCLEIC ACID NANOTECHNOLOGY FOR GENETIC REGULATION IN VIVO
12.1 Introduction
12.2 Toehold-mediated strand displacement: switching nucleic acids with nucleic acids
12.3 Toehold riboregulators and related systems
12.4 Applying nucleic acid nanotechnology to CRISPR and RNA interference
12.5 Delivery of nucleic acid devices, in vivo production, and challenges for in vivo operation
12.6 Conclusion & Outlook

13. CELL MEMBRANE FUNCTIONALIZATION VIA NUCLEIC ACID TOOLS FOR VISUALIZATION AND REGULATION OF CELLULAR RECEPTORS
13.1 Nucleic Acid-based Functionalization Strategies: From Receptor Information to