Mononuclear Non-heme Iron Dependent Enzymes

After completing studies for the A.B., A.M., and Ph.D. degrees in chemistry at Harvard University, David W. Christianson joined the faculty of the University of Pennsylvania, where he is currently the Roy and Diana Vagelos Professor in Chemistry and Chemical Biology. At Penn, Christianson’s research focuses on the structural and chemical biology of the zinc-dependent histone deacetylases as well as enzymes of terpene biosynthesis. His research accomplishments have been recognized by several awards, including the Pfizer Award in Enzyme Chemistry and the Repligen Award in Chemistry of Biological Processes from the American Chemical Society, a Guggenheim Fellowship, and the Elizabeth S. and Richard M. Cashin Fellowship from the Radcliffe Institute for Advanced Study at Harvard University. Christianson is also a dedicated classroom teacher, and his accomplishments in this regard have been recognized by the Lindback Award for Distinguished Teaching at Penn and a Rhodes Trust Inspirational Educator Award from Oxford University. Christianson has also held visiting professorships in the Department of Biochemistry at Cambridge University and the Department of Chemistry and Chemical Biology at Harvard University. Christianson has served with Prof. Anna Pyle as Co-Editor-in-Chief of Methods in Enzymology since 2015.

1. Photoreduction for Rieske oxygenase chemistry
Pinghua Liu
2. Insights into the Mechanisms of Rieske Oxygenases from Studying the Unproductive Activation of Dioxygen
Thomas B. Hofstetter, Charlotte E. Bopp and Nora M. Bernet
3. Non-heme iron and 2-oxoglutarate enzymes catalyze cyclopropane and azacyclopropane formations
Wei-chen Chang
4. Obtaining precise metrics of substrate positioning in Fe(II)/2OG dependent enzymes using Hyperfine Sublevel Correlation Spectroscopy
Alexey Silakov
5. Xe-pressurization studies for revealing substrate-entrance tunnels
Jennifer D. Bridwell-Rabb
6. A tale of two dehydrogenases involved in NADH recycling
Jennifer D. Bridwell-Rabb
7. Rieske oxygenases and/or their partner reductase proteins
Pravindra Kumar
8. Expression, assay and inhibition of 9-cis-epoxycarotenoid dioxygenase (NCED) from Solanum lycopersicum and Zea mays
Timothy David Bugg
9. Biocatalysis and non-heme iron enzymes
Xiongyi Huang
10. In vitro analysis of the three-component Rieske oxygenase cumene dioxygenase from Pseudomonas fluorescens IP01
Sandy Schmidt and Niels A.W. de Kok
11. Structure and function of carbazole 1,9a-dioxygenase
Hideaki Nojiri
12. Characterization of a Mononuclear Nonheme Iron-dependent Mono-oxygenase OzmD in Oxazinomycin Biosynthesis
Hung-wen Liu
13. Spectroscopic and computational studies of a bifunctional iron- and 2-oxoglutarate dependent enzyme, AsqJ
Yisong Guo
14. Non-heme iron oxygenases for natural product biosynthesis
Ikuro Abe
15. Isonitrile Formation by a Non-Heme Iron(II)-Dependent Oxidase/Decarboxylase
Wenjun Zhang
16. Assays for the epidermal growth factor hydroxylase Asph
Christopher Joseph Schofield
17. Amino acid hydroxylases
Paul Fitzpatrick
18. Extradiol dioxygenases
Yifan Wang
19. Title to be determined
Ramaswamy Subramanian
20. Experimental and computational approaches to understanding and addressing the stability limitations of Rieske Oxygenases
Jennifer DuBois
21. Construction and Testing of Whole-Cell Non-Heme Iron Biocatalysts
Ryan Summers
22. Equilibrium dialysis to measure binding affinities of non-heme iron enzymes
Ambika Bhagi-Damodaran
23. title to be determined
Thomas C. Brunold
24. Approaches to determination of the mechanism of the Rieske monooxygenase salicylate 5-hydroxylase
John D. Lipscomb and Melanie Rogers
25. Preparation of reductases for multicomponent oxygenases
Lindsay David Eltis and Megan Wolf
26. Expression, purification, and biochemical characterization of micro- and macroalgal kainoid synthases
Shaun McKinnie
27. Title to be determined
Kabirul Islam
28. Title to be determined
Edward I. Solomon
29. title to be detrmined
Adam Offenbacher