Receptor Signal Transduction Protocols

Methods for Characterizing Receptors.- Radioligand-Binding Methods for Membrane Preparations and Intact Cells.- Site-Directed Mutagenesis and Chimeric Receptors in the Study of Receptor-Ligand Binding.- Approaches to the Stable Transfection of G Protein-Coupled Receptors.- Methods for Transient Expression of Hetero-Oligomeric Ligand-Gated Ion Channels.- The Generation of Receptor-Selective Antibodies.- In Situ Hybridization.- lmmunocytochemical Methods for Investigating Receptor Localization.- Methods for Studying Receptor Coupling Mechanisms.- Measurement of Agonist-Stimulated [35S]GTP?S Binding to Cell Membranes.- Autoradiographic Visualization in Brain of Receptor-G Protein Coupling Using [35S]GTP?S Binding.- Agonist-Induced High-Affinity GTP Hydrolysis as an Index of Receptor-Mediated G Protein Activation in Mammalian Brain Membranes.- Use of Random-Saturation Mutagenesis to Study Receptor-G Protein Coupling.- Identification and Quantitation of G Protein ?-Subunits.- Covalent Modification of G Proteins by Affinity Labeling.- Heterologous Expression of Receptors and Signaling Proteins in Adult Mammalian Sympathetic Neurons by Microinjection.- Nuclear Application of Antisense Oligonucleotides by Microinjection and Ballistomagnetic Transfer to Identify G Protein Heterotrimers Activating Phospholipase C.- Use of Antisense-Generating Plasmids to Probe the Function of Signal Transduction Proteins in Primary Neurons.- Methods for Investigating the Regulation of Receptor Function.- Protocols Employed in the Investigation of G Protein-Coupled Receptor Phosphorylation.- Assay of G Protein-Coupled Receptor Kinase Activity by Rhodopsin Phosphorylation.- Radioligand Binding Measurement of Receptor Sequestration in Intact and Permeabilized Cells.- Regulation of ReceptorExpression.