Receptor Signal Transduction Protocols

Methods for Characterizing Receptors. Radioligand-Binding Methods for Membrane Preparations and Intact Cells Mary Keen. Site-Directed Mutagenesis and Chimeric Receptors in the Study of Receptor-Ligand Binding Mark E. Olah and Gary L. Stiles. Approaches to the Stable Transfection of G Protein-Coupled Receptors Andrea Townsend-Nicholson. Methods for Transient Expression of Hetero-Oligomeric Ligand-Gated Ion Channels Hartmut L?ddens and Esa R. Korpi. The Generation of Receptor-Selective Antibodies John J. Mackrill. In Situ Hybridization Judith C.W. Mak and Peter J. Barnes. Immunocytochemical Methods for Investigating Receptor Localization Maria I. Fonseca and R. Dale Brown. Methods for studying receptor coupling mechanisms. Measurement of Agonist-Stimulated [35S]GTPgS Binding to Cell Membranes Sebastian Lazareno. Autoradiographic Visualization in Brain of Receptor-G Protein Coupling Using [35S]GTPgS Binding Laura J. Sim Dana E. Selley and Steven R. Childers. Agonist-Induced High-Affinity GTP Hydrolysis As an Index of Receptor-Mediated G Protein Activation in Mammalian Brain Membranes Yuji Odagaki and Kjell Fuxe. Use of Random-Saturation Mutagenesis to Study Receptor-G Protein Coupling Ethan S. Burstein Tracy A. Spalding and Mark R. Brann. Identification and Quantitation of G Protein a-Subunits Ian Mullaney and Graeme Milligan. Covalent Modification of G Proteins by Affinity Labeling Martin Hohenegger Michael Freissmuth and Christian Nanoff. Heterologous Expression of Receptors and Signaling Proteins in Adult Mammalian Sympathetic Neurons by Microinjection Stephen R. Ikeda. Nuclear Application of Antisense Oligonucleotides by Microinjection and Ballistomagnetic Transfer to Identity G Protein Heterotrimers Activating Phospholipase C Frank Kalkbrenner Edgar Dippel Matthias Schroff Burghardt Wittig and G?nter Schultz. Use of Antisense-Generating Plasmids to Probe the Function of Signal Transduction Proteins in Primary Neurons Fe C. Abogadie Yvonne Vallis Noel J. Buckley and Malcolm P. Caulfield. Methods for investigating the regulation of receptor function. Protocols Employed in the Investigation of G Protein-Coupled Receptor Phosphorylation Andrew B. Tobin. Assay of G Protein-Coupled Receptor Kinase Activity by Rhodopsin Phosphorylation Alison W. Gagnon and Eamonn Kelly. Radioligand Binding Measurements of Receptor Sequestration in Intact and Permeabilized Cells Diana M. Slowiejko and Stephen K. Fisher. Regulation of Receptor Expression: Analysis of Receptor mRNA and Gene Transcription Philip A. Iredale Maura E. Charlton John D. Alvaro and Ronald S. Duman. Index.