Receptor Binding Techniques
Humana Press Inc. (Verlag)
978-0-89603-530-0 (ISBN)
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This cutting-edge collection of step-by-step experimental protocols demonstrates the power of radioligand binding for studying a wide variety of receptors. The methods can be applied to G protein coupled receptors, receptors with integral ion channels, and sites other than receptors, such as guanine nucleotide binding proteins, enzymes, transporters, and mRNA. The individual protocols encompass many different preparations, ranging from solubilized receptors, through membrane preparations, intact cells, and autoradiography in tissue slices, to PET and SPECT analysis of binding in living human brain. Along with the detailed, easily reproducible instructions, the protocols also address the problems inherent in the basic binding technique, such as common experimental artifacts and the definition of receptor sites, as well as problems associated with the use of particular ligands and receptor preparations. Receptor Binding Techniques shows clearly how these methods can be adapted to many different applications and fully utilized in drug discovery programs.
Part I. Introduction. Overview of Ligand-Receptor Binding Techniques M. Qume. Part II. Radioligand Binding in Different Preparations. [3H]-GABA binding to GABAA and GABAB Sites on Rat Brain Crude Synaptic Membranes R. Bruton and M. Qume. Characterization of Imidazoline Receptors by Radioligand Binding A. L. Hudson and L. A. Lione. Radioligand Binding to Solubilized 5 HT3 Receptors S. Fletcher and N. M. Barnes. Solubilized Muscarinic Acetylcholine Receptors from the Rat Myocardium: Pharmacological and Hydrodynamic Characterization C. P. Berrie and M. Keen. Radioligand Binding in Intact Cells J. A. Koenig. Autoradiography of Peptide Receptors J. Wharton and D. A. Walsh. Measurement of Brain GABA-Benzodiazepine Receptor Levels in Vivo Using Emission Tomography A. Lingford-Hughes and A. Malizia. Part III. General Considerations. Analysis of Binding Data E. C. Hulme. Definition of Receptors in Radiogland Binding Experiments M. Keen. Part IV. The Use of Binding to Study NonReceptor Sites. Autoradiography of Enzymes Second Messenger Systems and Ion Channels D. A. Walsh and J. Wharton. Whole Human Brain Autoradiography of Serotonin Re-Uptake Inhibitors M. Qume and J. K. Miller. Measurement of Agonist-Stimulated [35S] GTPgS Binding to Cell Membranes S. Lazareno. mRNA: Detection by In Situ and Northern Hybridization R. M. C. Parker and N. M. Barnes. Index.
| Reihe/Serie | Methods in Molecular Biology |
|---|---|
| Verlagsort | Totowa, NJ |
| Sprache | englisch |
| Maße | 229 x 152 mm |
| Gewicht | 658 g |
| Themenwelt | Naturwissenschaften ► Biologie ► Biochemie |
| Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie | |
| ISBN-10 | 0-89603-530-1 / 0896035301 |
| ISBN-13 | 978-0-89603-530-0 / 9780896035300 |
| Zustand | Neuware |
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