Methods for Monitoring Mitochondrial Functions During Pathogen Infection

Saverio Marchi obtained his PhD in ‘Pharmacology and Molecular Oncology’ from University of Ferrara (Italy), under the supervision of Prof. Rosario Rizzuto, where he investigated the role of Ca2+ signalling and mitochondrial dynamics in cancer. He did a short term research stay at the Gustave Roussy Institute (Paris, France) in the Guido Kroemer’s lab, for the study of the mechanisms of autophagy regulation. On 2011 he joined the Laboratory of Dr. Paolo Pinton at the University of Ferrara (Italy) where he carried out his postdoctoral studies. During his postdoctoral training, he studied the implications of Endoplasmic Reticulum-mitochondria connection in diseases, especially cancer. In 2014, he was the recipient of the Young Researcher award, which allowed him to work at the Cancer Center, University of Hawai’i (Prof. Michele Carbone’s lab), where he was introduced to the study of the signalling pathways regulating mesothelioma progression. Now, he is associate professor at the Marche Polytechnic University, where he leads his own group. He is interested in the involvement of the MCU complex and mitochondrial Ca2+ in the evolution of cancer. More recently, he has become interested in the alteration of mitochondrial dynamics during bacterial infection. He published 77 papers on “peer-reviewed” journals (h-index: 41; n° of citations > 5800. Source: Scopus). Lorenzo Galluzzi is Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College, Honorary Assistant Professor Adjunct with the Department of Dermatology of the Yale School of Medicine, Honorary Associate Professor with the Faculty of Medicine of the University of Paris, and Faculty Member with the Graduate School of Biomedical Sciences and Biotechnology of the University of Ferrara, the Graduate School of Pharmacological Sciences of the University of Padova, and the Graduate School of Network Oncology and Precision Medicine of the University of Rome “La Sapienza”. Moreover, he is Associate Director of the European Academy for Tumor Immunology and Founding Member of the European Research Institute for Integrated Cellular Pathology. Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. He has published over 450 articles in international peer-reviewed journals and is the Editor-in-Chief of four journals: OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, Methods in Cell biology, and Molecular and Cellular Oncology (which he co-founded in 2013). Additionally, he serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease, Pharmacological Research and iScience.

1. Mitochondrial Labeling with Fluorescent Proteins for Enhanced Morphological Characterization and Visualization of Intercellular Mitochondrial Transfer
Toshio Miki
2. Visualizing mitochondrial electron transport chain complexes and super-complexes during infection of human macrophages with Legionella pneumophila
Pedro Escoll
3. Quantitative assessment of mitochondrial membrane potential in macrophages in sepsis
Guochang Hu
4. Quantification of mitochondrial reactive oxygen species in macrophages during sepsis
Guochang Hu
5. Quantification of intracellular and mitochondrial ATP content in macrophages during lipopolysaccharide-induced inflammatory response
Guochang Hu
6. Analysis of cytosolic mtDNA release during Staphylococcus Aureus infection
Saverio Marchi
7. Monitoring mitochondria dynamics upon infection using a tomographic approach
Gianluca Morroni
8. A screening system to determine bacterial effects on MADCAM1 expression by high endothelial venule cells
Oliver Kepp
9. Isolation of mitochondria from Mycobacteria infected cells
Krishnaveni Mohareer
10. Monitoring mitochondrial localization of Mycobacterial proteins
Krishnaveni Mohareer
11. Bacterial predators and BALOs: growth protocol and relation with mithochondria
Valerio Iebba
12. Assessment of adhering and invading properties of Escherichia coli strains
Valerio Iebba
13. Bioinformatic techniques to study intracellular bacteria
Valerio Iebba