Vectorology for Optogenetics and Chemogenetics

Production, testing and verification of Lentivirus for regional targeting in the old-world monkey brain.- HiRet/NeuRet vectors: Lentiviral system for highly efficient gene transfer through retrograde axonal transport.-Generation of High-Titer Defective HSV-1 Amplicon Vectors.- Generation and Application of Engineered Rabies Viral Vectors for Neural Circuit Research.- Quality control for Adeno-associated viral vector production.- Vector tropism.-Viruses for systemic delivery.- Transcriptomic Definition of Neuron Types.- Enhancers for selective targeting.- Pathway-selective reversible perturbations using a double-infection technique in the macaque brain.- Pathway-Specific Chemogenetic Manipulation by Applying Ligand to Axonally-Expressed DREADDs.- Convection-enhanced delivery of viral vectors.- Multi-channel microinjector arrays for efficient viral vector delivery into rhesus monkey brain.- Methods to verify expression and function of DREADDs using PET.- Reporter selection and post-mortem methods to verify transgene expression.- Considerations for the use of viral vectors in NHP experiments.