Thermomorphogenesis

A Guide to Quantify Arabidopsis Seedling Thermomorphogenesis at Single Timepoints and by Interval Monitoring.- Dissection of Daytime and Nighttime Thermoresponsive Hypocotyl Elongation in Arabidopsis.- Light-Dependent High Ambient Temperature-Induced Senescence Assay Using Whole Seedlings.- Assessing Temperature Responses in Roots.- A Luciferase Imaging-Based Assay for Studying Temperature Compensation of the Circadian Clock.- Investigating the Effects of Temperature on Pathogen Propagation in Arabidopsis.- Assessing the High Temperature Effects on Stomatal Production.- Characterizing the Synergistic Response Induced by Warm Temperatures and Shade.- Measurement of Phytochrome B Thermal Reversion Rates In Vivo.- Characterization of Thermoresponsive Photobody Dynamics.- Phase Separation of Phytochrome B in HEK293T Cells.- Isolation of Phytochrome B Photobodies.- Analysis of Phase Separation of EARLY FLOWERING 3.- In Vitro Determination of Temperature-Dependent DNA-Binding of the Evening Complex Using Electrophoretic Mobility Shift Assays.- Monitoring Real-Time Temperature Dynamics of a Short RNA Hairpin Using Förster Resonance Energy Transfer and Circular Dichroism.- Characterization of PIF4 Phosphorylation by SPA1.- Chromatin Immunoprecipitation to Investigate H2A.Z Dynamics in Response to Environmental Changes.- Assessing the Function of CBF1 in Modulating the Interaction between Phytochrome B and PIF4.- GST Pull-Down Assay to Study PIF4 Binding In Vitro.- Investigating Circadian Gating of Temperature Responsive Genes.- RuBisCO Depletion and Subcellular Fractionation for Enhanced Florigen Detection in Arabidopsis.- Identifying Causal Genes for Thermo-Responsive Rosette Growth in Arabidopsis by Genome-Wide Association Study.- Mathematical Modeling of Photo- and Thermomorphogenesis in Plants.