New Technologies for Glutamate Interaction

Visualization of Glutamatergic Neurotransmission in Diverse Model Organisms with Genetically Encoded Indicators.- Surface Glutamate Receptors Nanoscale Organization with Super-Resolution Microscopy (dSTORM).- Ligand-Directed Chemical Labeling for Visualizing and Analyzing AMPA Receptors in Neurons.- Live FRET-FLIM Imaging to Study Metabotropic Signaling via the NMDA Receptor.- Quantitative Analysis of Single Glutamatergic Vesicles in the Brain.- Imaging Microtubule Network in Rodent Giant Glutamatergic Presynaptic Terminals.- Photooxidation of Genetically Encoded MiniSOG-Fused VGLUT2 for Identification of Glutamatergic Synapses by Transmission and 3D Electron Microscopy.- Chemogenetic Approaches to Study Astrocytes at Glutamatergic Synapses.- Studying the Role of Astrocytes at Synapses using Single-Cell Transcriptomics.- Analysis of Synaptic Glutamate Clearance as a Possible Indicator of Synaptic Health in the Degenerating Rodent Brain.- Computational Models of Astrocyte Function at Glutamatergic Synapses.- Electrophysiological Recordings of Oligodendroglia in Adult Mouse Brain Slices.- Synaptic Integration at Neuron-OPC Synapses.- Mapping Synaptic Inputs to Oligodendroglial Cells using In Vivo Monosynaptic Viral Tracing.- In Vivo Viral Gene Delivery to Manipulate Functional Properties of AMPA Receptors in Oligodendrocyte Lineage Cells.- Studying Synaptic Integration of Glioma Cells into Neural Circuits.