Preservation and Maintenance of Living Fungi

David Smith has 44 years at CABI and is currently Director of Biological Resources with a long history of managing a living fungal collection; developing and managing projects on conservation and use of microorganisms having presented over 150 conference papers and 180 publications, including 2 books and 25 book chapters.

1: Foreword 2: Microbial resource collections: aims, management and information 3: Collection management 4: Acquisition of strains 5: Accession 6: Preservation and storage 7: Culture properties 8: Distribution 9: Information 10: Continuous growth and adaptations for storage 11: Growth 12: Methods of culture maintenance 13: Sub-culturing 14: Storage under mineral oil 15: Water storage 16: Summary of maintenance by growth techniques 17: Drying and freeze-drying 18: Drying 19: Silica gel storage 20: Soil storage 21: Freeze-drying 22: Factors affecting survival in freeze-drying 23: Cryopreservation 24: Method 25: Optimization of preservation conditions 26: Selection of preservation techniques 27: Recommended methods of preservation for taxonomic groups 28: Selection of preservation technique based upon collection requirements 29: Comparison of methods available 30: Suitability of the techniques for fungi 31: Summary 32: Special techniques and procedures 33: Cleaning and mite prevention 34: Discarding cultures 35: Cryogenic light microscopy 36: Means of achieving reproducible cooling rates