Chemokine-Glycosaminoglycan Interactions

A Surgical Approach to Hindlimb Suspension: A Mouse Model of Disuse-Induced Atrophy.- Remote Ischemic Pre-Conditioning (RIPC).- Mouse Corneal Transplantation.- Analysis of Chemokine-to-GAG Interactions in Model of Donor Renal Allograft Transplant.- Mouse Models of Renal Allograft Transplant Rejection: Methods to Investigate Chemokine:GAG Interaction and Therapeutic Blockade.- Ex Vivo Perfusion System to Analyze Chemokine-Driven Leukocyte Adhesion.- In Vitro (Trans)migration Experiment Using Chemokines as Stimulatory Factor.- The Use of Campenot Trichambers for the Study of Peripheral Neuronal Growth and Survival in Presence of Thrombotic Factors and Serpins.- Flow Cytometry Analysis of Immune Cell Responses.- Detection of Chemokine Binding Proteins Association to Cell Surface Glycosaminoglycans by Flow Cell Cytometry and Indirect Immunofluorescence.- Protein Microarrays and Their Fabrication.- NMR Methods for Characterization of Glycosaminoglycan-Chemokine Interactions.- Glycosaminoglycan Analysis: Purification, Structural Profiling, and GAG-Protein Interactions.- Isolation and Compositional Analysis of Glycosaminoglycans.- How to Design Peptides.- Methods to Assess Chemokine Binding and Anti-Chemotactic Activity of Virus Proteins.- Producing Biologics with Defined N-Glycosylation in Plants.- Native Deglycosylation and Size Exclusion Chromatography of Viral Chemokine Binding Proteins for Structural Discovery.