Myeloid-Derived Suppressor Cells -

Myeloid-Derived Suppressor Cells

Buch | Hardcover
318 Seiten
2024
Academic Press Inc (Verlag)
978-0-443-21807-1 (ISBN)
169,95 inkl. MwSt
Myeloid-Derived Suppressor Cells, Volume 184 in the Methods in Cell Biology series includes comprehensive protocols to assess the role of myeloid-derived suppressor cells (MDSCs), an immature, heterogenous cell population from the myeloid lineage with a potent immunosuppressive activity in a variety of diseases. This volume includes interesting protocols that address the Detection of myeloid-derived suppressor cells by flow cytometry, Determination of murine myeloid-derived suppressor cells by flow cytometry, Isolation of Myeloid-Derived Suppressor Cells (MDSC) from Endometriotic Mice Model and their Immunomodulatory Functions, Simple protocol for measuring CD11b+ GR-1+ (Ly6C+/Ly6G+) myeloid cells from a minimum volume of mouse peripheral blood, and much more.

Additional chapters cover Assessment of myeloid-derived suppressor cell differentiation ex vivo, Single clonal tracking on biomimetic microtextured platforms for real-time guided migration analysis of myeloid-derived suppressor cell dissemination characteristics ex vivo, Isolation and immunosuppressive functions of myeloid-derived suppressor cell-derived exosomes, Characterization of lysosomal acid lipase in Ly6G+ and CD11c+ myeloid-derived suppressor cells, In Vitro Osteoclastogenesis Assessment using Murine Myeloid-derived Suppressor Cells, Estimating Nitric Oxide (NO) from MDSCs by Griess Method, and In vitro generation of myeloid-derived suppressor cells (MDSCs) from hematopoietic progenitor cells (HPCs).

Carlos Jiménez-Cortegana obtained the degree in Chemistry at University of Seville (Spain) in 2014. In July 2020 he got the PhD in Clinical Research at the same University, belonging to the group headed by Dr. Luis de la Cruz-Merino and Dr. Víctor Sánchez-Margalet. Currently, Carlos is a Postdoctoral Researcher at the Department of Radiation Oncology, Weill Cornell Medical College (New York, USA), in the research group headed by Dr. Lorenzo Galluzzi. From 2021, Carlos has published more than 15 articles in recognized, international journals, some of them in high-impact factor journals, mainly focused on the immunosuppressive role of myeloid-derived suppressor cells in a variety of diseases, including breast cancer, diffuse large B-cell lymphoma, and COVID-19. Lorenzo Galluzzi is Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College, Honorary Assistant Professor Adjunct with the Department of Dermatology of the Yale School of Medicine, Honorary Associate Professor with the Faculty of Medicine of the University of Paris, and Faculty Member with the Graduate School of Biomedical Sciences and Biotechnology of the University of Ferrara, the Graduate School of Pharmacological Sciences of the University of Padova, and the Graduate School of Network Oncology and Precision Medicine of the University of Rome “La Sapienza”. Moreover, he is Associate Director of the European Academy for Tumor Immunology and Founding Member of the European Research Institute for Integrated Cellular Pathology. Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. He has published over 450 articles in international peer-reviewed journals and is the Editor-in-Chief of four journals: OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, Methods in Cell biology, and Molecular and Cellular Oncology (which he co-founded in 2013). Additionally, he serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease, Pharmacological Research and iScience.

1. Detection of myeloid-derived suppressor cells by flow cytometry
Tianju Liu
2. Determination of murine myeloid-derived suppressor cells by flow cytometry
Hiroshi Kitamura
3. Isolation of Myeloid-Derived Suppressor Cells (MDSC) from Endometriotic Mice Model and their Immunomodulatory Functions
Gene Man
4. Simple protocol for measuring CD11b+ GR-1+ (Ly6C+/Ly6G+) myeloid cells from a minimum volume of mouse peripheral blood
Gabriel Cabrera
5. CD4+ T cells proliferation assays to analyze Mo-MDSC suppressive function
Marcos López-Hoyos
6. Assessment of myeloid-derived suppressor cell differentiation ex vivo
Ester Blanco
7. Single clonal tracking on biomimetic microtextured platforms for real-time guided migration analysis of myeloid-derived suppressor cell dissemination characteristics ex vivo
Daniel Gallego-Pérez
8. Isolation and immunosuppressive functions of myeloid-derived suppressor cell-derived exosomes
Ali Syed Arbab
9. Characterization of lysosomal acid lipase in Ly6G+ and CD11c+ myeloid-derived suppressor cells
Ting Zhao and Hong Du
10. In Vitro Osteoclastogenesis Assessment using Murine Myeloid-derived Suppressor Cells
KYU HWAN KWACK and Keith Lough Kirkwood
11. Estimating Nitric Oxide (NO) from MDSCs by Griess Method
Saikat Mukherjee
12. In vitro generation of myeloid-derived suppressor cells (MDSCs) from hematopoietic progenitor cells (HPCs)
Kevin Alicea-Torres

Erscheinungsdatum
Reihe/Serie Methods in Cell Biology
Verlagsort San Diego
Sprache englisch
Maße 191 x 235 mm
Gewicht 450 g
Themenwelt Naturwissenschaften Biologie Genetik / Molekularbiologie
Naturwissenschaften Biologie Zellbiologie
ISBN-10 0-443-21807-2 / 0443218072
ISBN-13 978-0-443-21807-1 / 9780443218071
Zustand Neuware
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