Translational Research Methods in Neurodevelopmental Disorders

Using Lentiviral shRNA Delivery to Knock-Down Proteins in Cultured Neurons and In Vivo.- Synaptosomal Preparation and Its Application in Preclinical Studies.- Assessing the Nanoscale Organization of Excitatory and Inhibitory Synapses using Recombinant Probes to Visualize Endogenous Synaptic Proteins.- Agonist-Induced Functional Analysis and Cell Sorting (aiFACS) to Select and Characterize Interneurons during Brain Development.- A CRISPR/Cas9-Based Toolkit to Test Gain- and Loss-of-Gene Function in Brain Organoids.- Assessment of Adult Mouse Brain NeuroAnatomical Phenotypes using Quantitative and Precision Histology.- Leveraging Microelectrode Array Technology for Phenotyping Stem Cell-Derived Neurodevelopmental Disease Models.- Humanized Chimeric Mouse Models to Study Human Neural Development and Pathogenesis of Brain Diseases.- In Vivo Manipulations to Correct for Behavioral Phenotypes in Cognitive Disorder Mouse Models.- Assessing Dyadic Social Interactions in Rodent Models of Neurodevelopmental Disorders.- Central and Peripheral Explorations by Metabolomics to Study Neurodevelopmental Disorders.- Genetic Engineering of Non-Human Primates Models for Studying Neurodevelopmental Disorders.