Wolbachia

Wolbachia:  Advancing into a second century.- Investigation of cytoplasmic incompatibility patterns in Culex pipiens: from field samples to laboratory isofemale lines.- Maintenance and Evaluation of Wolbachia Male-Killers of Dipterans.- Examining Wolbachia-Induced Parthenogenesis in Hymenoptera.- Pillbug: a toolbox for feminisation.- Detection, Quantification and Elimination of Wolbachia in Bed Bugs.- Detection and analysis of Wolbachia in plant-parasitic nematodes and insights into Wolbachia evolution.- Developing Wolbachia-infected cell lines from insects.- Use of antibiotics to eliminate Wolbachia from mosquitos and cell culture.- Preparation of infectious Wolbachia from a mosquito cell line.- Wolbachia transinfection via embryonic microinjection.- Measuring host fitness effects and transmission of Wolbachia strains in Aedes aegypti mosquitoes.- Detection of natural Wolbachia strains in Anopheles mosquitoes.- Procedures for detection of Wolbachia-conferred antiviral protection in Drosophila melanogaster.- Validating a mitochondrial sweep accompanying the rapid spread of a maternally-inherited symbiont.- Screening host genomic data for Wolbachia infections.- A worked example of screening genomic material for the presence of Wolbachia infection.- Use of Drosophila Transgenics to Identify Functions for Symbiont Effectors.- Using Baker’s Yeast to Determine Functions of Novel Wolbachia (and other prokaryotic) Effectors.- Isolation of Phage WO Particles from Wolbachia-infected Arthropods.- A light in the dark: Uncovering Wolbachia-host interactions using fluorescence imaging.