Platelets

Preface ; Acknowledgement ; 1. Platelet preparation and functional responses ; 2. Cellular model systems to study megakaryocyte differentiation ; 3. Techniques in the preparation of semi-permeable platelets and their uses ; 4. Use of fluorescent indicators to measure intracellular Ca2+ and other ions ; 5. Preparation of highly purified human platelet plasma and intracellular membranes using high voltage free flow electrophoresis and methods to study Ca2+ regulation ; 6. The use of flow-cytometry to study platelet activation ; 7. Identification, isolation, and characterization of platelet glycoproteins mediating platelet adhesion ; 8. Monoclonal antibodies to platelet cell surface antigens and their use ; 9. Measurement of protein phosphorylation, kinase activity, and G protein function in intact platelets and membrane preparations ; 10. Tyrosine phosphorylation ; 11. Study of proteins associated with the platelet cytoskeleton ; 12. Methods for measuring agonist-induced phosphlipid metabolism in intact human platelets ; 13. Cyclic nucleotides: measurement and function ; 14. Immunocytochemical and electron microscope studies of platelets ; 15. Clinical aspects of platelet function ; 16. Platelet procoagulent activity and its measurement ; 17. The study of platelet function in other species ; List of suppliers ; Index