Reactive Oxygen Species in Plants

lt;p>Part I: Strategies to Induce ROS Production

1. Modification of Chloroplast Antioxidant Capacity by Plastid Transformation

            Shengchun Li, Pan Shen, Bipeng Wang, Xiujie Mu, Mimi Tian, Tao Chen, and Yi Han

2. Analysis of Ascorbate Metabolism in Arabidopsis under High-Light-Stress

            Takanori Maruta and Takahiro Ishikawa

3. Genetic Manipulation of Reactive Oxygen Species (ROS) Homeostasis Utilizing CRISPR/Cas9-Based Gene Editing in Rice

            Sheng Xu, Tao Chen, Mimi Tian, Marie-Sylviane Rahantaniaina, Linlin Zhang, Ren Wang, Wei Xuan, and Yi Han

4. Studying Plant Stress Reactions In Vivo by PAM Chlorophyll Fluorescence Imaging

            Alexey Shapiguzov and Jaakko Kangasjärvi

Part II: Methods to Visualize ROS and to Detect Changes in Redox Homeostasis

5. Live Monitoring of ROS-Induced Cytosolic Redox Changes with roGFP2-Based Sensors in Plants

            José Manuel Ugalde, Lara Fecker, Markus Schwarzländer, Stefanie J. Müller-Schüssele, and Andreas J. Meyer

6. Quantitative Measurement of Ascorbate and Glutathione by Spectrophotometry

            Graham Noctor and Amna Mhamdi

7. Measurement of NAD(P)H and NADPH-Generating Enzymes

            Amna Mhamdi, Frank Van Breusegem, and Graham Noctor

8. Quantitative Analysis for ROS-Producing Activity and Regulation of Plant NADPH Oxidases in HEK293T Cells

            Sachie Kimura, Hidetaka Kaya, Kenji Hashimoto, Michael Wrzaczek, and Kazuyuki Kuchitsu

Part III: Small-Scale Targeted Analysis of ROS Accumulation during Stress and Effects on Plant Physiology

9. Estimation of the Level of Abasic Sites in Plant mRNA Using Aldehyde Reactive Probe

            Jagna Chmielowska-Bak, Karolina Izbianska-Jankowska, and Joanna Deckert

10. A Simplified Method to Assay Protein Carbonylation by Spectrophotometry

            Corentin Moreau and Emmanuelle Issakidis-Bourguet

11. In Vitro Biochemical Analysis of Recombinant Plant Proteins under Oxidation

            Zeya Chen and Jingjing Huang

12. Methods to Analyze the Redox Reactivity of Plant Proteins

            Thualfeqar Al-Mohanna, George V. Popescu, and Sorina C. Popescu

13. Determination of ROS-Induced Lipid Peroxidation by HPLC-Based Quantification of Hydroxy Polyunsaturated Fatty Acids

            Brigitte Ksas and Michel Havaux

14. Detection of Lipid Peroxidation-Derived Free Azelaic Acid, a Biotic Stress Marker and Other Dicarboxylic Acids in Tobacco by Reversed Phase HPLC-MS under Non-Derivatized Conditions

            Attila L. Ádám, György Kátay, András Künstler, and Lóránt Király

15. Determination of Reactive Carbonyl Species, which Mediate Reactive Oxygen Species Signals in Plant Cells

            Jun'ichi Mano, Md. Sanaullah Biswas, Koichi Sugimoto, and Yoshiyuki Murata

16. Measuring Stress-Induced Changes in Defense Phytohormones and Related Compounds

Part IV: Systems Biology Approaches to Understand ROS Functions

17. Targeted Mass Spectrometry Analysis of Protein Phosphorylation by Selected Ion Monitoring Coupled to Parallel Reaction Monitoring (tSIM/PRM)

            Jesús Pascual and Saijaliisa Kangasjärvi

18. Quantitative Analysis of Post-Translational Modifications of Plant Histones

            Hana Kucharíková, Zuzana Plsková, Zbynek Zdráhal, Miloslava Fojtová, Pavel Kerchev, and Gabriela Lochmanová

19. Characterization of RBPome in Oxidative Stress Conditions

            Zhicheng Zhang, Evy Timmerman, Francis Impens, and Frank Van Breusegem

20. Analysis of ROS-Triggered Changes in the Transcriptome