SH2 Domains

Methods for Structure Determination of SH2 Domain–Phosphopeptide Complexes by NMR.- NMR Methods to Study the Dynamics of SH2 Domain–Phosphopeptide Complexes.- Crystal Structure Analysis of SH2 Domains in Complex with Phosphotyrosine Peptides.- Revealing Allostery in PTPN11 SH2 Domains from MD Simulations.- Structure Determination of SH2–Phosphopeptide Complexes by X-Ray Crystallography: The Example of p120RasGAP.- Fluorescence Anisotropy and Polarization in the Characterization of Biomolecular Association Processes and Their Application to Study SH2 Domain Binding Affinity.- Computational Evaluation of Peptide-Protein Binding Affinities: Application of Potential of Mean Force Calculations to SH2 Domains.- NMR Relaxation Dispersion Experiments to Study Phosphopeptide Recognition by SH2 Domains: The Grb2-SH2–Phosphopeptide Encounter Complex.- Using Linear Motif Database Resources to Identify SH2 Domain Binders.- Using Surface Plasmon Resonance to Study SH2 Domain-Peptide Interactions.- Inhibitor Library Screening of SH2 Domains through Denaturation-Based Assays.- Dissecting Selectivity Determinants of Small-Molecule Inhibitors of SH2-Domains via Fluorescence Polarization Assays.- Lipid Binding of SH2 Domains.- Exploring the Binding Interaction between Phosphotyrosine Peptides and SH2 Domains by Proximal Crosslinking.- Synthesis and Biochemical Evaluation of Monocarboxylic GRB2 SH2 Domain Inhibitors.- Engineering of SH2 Domains for the Recognition of Protein Tyrosine O-Sulfation Sites.- Engineering SH2 Domains with Tailored Specificities and Affinities.- Measuring Protein Tyrosine Phosphatase Activity Dependent on SH2 Domain-Mediated Regulation.- Peptides as Baits for the Co-Precipitation of SH2 Domain-Containing Proteins.- Biomolecular Condensation of SH2 Domain-Containing Proteins on Membranes.