Yeast Surface Display
Springer-Verlag New York Inc.
978-1-0716-2287-2 (ISBN)
Authoritative and practical, Yeast Surface Display serves as a comprehensive resource that enables the implementation of this powerful and versatile technique in virtually any molecular biology laboratory, even in the absence of any prior yeast surface display experience.
Yeast Surface Display: New Opportunities for a Time-Tested Protein Engineering System.- Yeast Surface Display for Protein Engineering: Library Generation, Screening, and Affinity Maturation.- Site-Wise Diversification of Combinatorial Libraries Using Insights from Structure-Guided Stability Calculations.- Ancestral Sequence Reconstruction and Alternate Amino Acid States Guide Protein Library Design for Directed Evolution.- Machine Learning-Driven Protein Library Design: A Path Toward Smarter Libraries.- Kinetic Competition Screening of Yeast-Displayed Libraries for the Generation of High Affinity Binders.- Engineering Proteins by Combining Deep Mutational Scanning and Yeast Display.- Engineering Binders with Exceptional Selectivity.- Affinity and Stability Analysis of Yeast Displayed Proteins.- Antibody Library Screening Using Yeast Biopanning and Fluorescence-Activated Cell Sorting.- A Hybrid Adherent/Suspension Cell-Based Selection Strategy for Discovery of Antibodies Targeting Membrane Proteins.- Ligand Selection by Combination of Recombinant and Cell Panning Selection Techniques.- Identification of Brain ECM Binding Variable Lymphocyte Receptors Using Yeast Surface Display.- Guidelines, Strategies, and Principles for the Directed Evolution of Cross-Reactive Antibodies Using Yeast Surface Display Technology.- Yeast Display for the Identification of Peptide-MHC Ligands of Immune Receptors.- Yeast Display Guided Selection of pH Dependent Binders.- Yeast Mating as a Tool for Highly Effective Discovery and Engineering of Antibodies via Display Methodologies.- Humanization of Chicken-Derived Antibodies by Yeast Surface Display.- Engineering Tissue Inhibitors of Metalloproteinases Using Yeast Surface Display.- Discovery of Cyclic Peptide Binders from Chemically Constrained Yeast Display Libraries.- Generation of Thermally Stable Affinity Pairs for Sensitive, Specific Immunoassays.- Isolating Anti-Amyloid Antibodies from Yeast-Displayed Libraries.- EngineeringProteins Containing Noncanonical Amino Acids on the Yeast Surface.- Construction of Yeast Display Libraries for Selection of Antigen-Binding Variants of Large Extracellular Loop of CD81, a Major Surface Marker Protein of Extracellular Vesicles.- Isolating and Engineering Fluorescence-Activating Proteins Using Yeast Surface Display.- Simultaneous Display of Multiple Kinds of Enzymes on the Yeast Cell Surface for Multistep Reactions.
Erscheinungsdatum | 03.05.2023 |
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Reihe/Serie | Methods in Molecular Biology |
Zusatzinfo | 96 Illustrations, color; 13 Illustrations, black and white; XV, 646 p. 109 illus., 96 illus. in color. |
Verlagsort | New York, NY |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Biochemie |
Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
Schlagworte | antibodies • Display libraries • Heterologous target protein anchoring • Mammalian Cells • Protein Engineering • Yeast-displayed protein variants |
ISBN-10 | 1-0716-2287-0 / 1071622870 |
ISBN-13 | 978-1-0716-2287-2 / 9781071622872 |
Zustand | Neuware |
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