Plant Tissue Culture: New Techniques and Application in Horticultural Species of Tropical Region

Prof. Dr. Duong Tan Nhut Dr. Duong Tan Nhut graduated from Kagawa University (Japan). He is the vice director and president of the Scientific Advisory Council of Tay Nguyen Institute for Scientific Research (VAST, Vietnam). He has more than four hundred peer-reviewed articles in international and national publications, and is the editor of the book Thin cell layer culture system: Regeneration and transformation application for Kluwer Academic Publishers; contributes approximately twenty book chapters for Springer, Kluwer Academic Publishers, as well as others; and has delivered numerous oral and poster presentations in numerous international meetings. Dr. Nhut was president of Biology – Agriculture – Life Sciences Council (NAFOSTED, Vietnam) in the years 2015–2017; is vice president of Vietnam Plant Physiology Association from 2003 to date; and is a member of editorial boards of certain journals in Vietnam.  Dr. Hoang Thanh Tung Dr. Hoang Thanh Tung graduated from Hue University of Sciences (Vietnam). He is a researcher at Tay Nguyen Institute for Scientific Research (VAST, Vietnam). Dr. Tung trained in histomorphology and histochemistry at Calgary University (Canada) and in the field of bio-industrial science at Tsukuba University (Japan). He has more than eighty-five peer-reviewed articles in international and national publications, has written two book chapters for Springer, and has delivered numerous oral and poster presentations in numerous national and international meetings. He was one of three nominees for the "Ta Quang Buu" Young Award presented by the Vietnam National Foundation for Science and Technology Development in 2020 and 2021. Prof. Dr. Edward C Yeung Dr. Edward C Yeung graduated from Yale University (USA). He is a professor at the Calgary University (Canada). He has more than two hundred fifty peer-reviewed international publications and has delivered numerous oral presentations at numerous international meetings. He is editorial board member of several international journals in plant physiology, biotechnology, and histology. He was awarded the University of Calgary Teaching Award in 2002 and is recipient of the Visiting Sr Scientist Award by the Japanese Society for the Promotion of Science in 2007.

Chapter 1. General information: some aspects of plant tissue culture.- Chapter 2. The use of the paraffin embedding method in the study of cultured explants I: Background information.- Chapter 3. The paraffin embedding method II: Protocols.- Chapter 4.  A simple guide to the use of compresstome in plant research.- Chapter 5. Establishment of nylon bag culture system in regeneration and micropropagation.- Chapter 6. Wounding manipulation and shoot tip removal methods in the micropropagation of Paphiopedilum callosum.- Chapter 7. Stem elongation for plant micropropagation.- Chapter 8. Somatic embryogenesis as a tool for propagating of some plants.- Chapter 9. Light-Emitting Diodes (LEDs) in plant regeneration, growth and secondary metabolite accumulation.- Chapter 10. In vitro hydroponic culture system in plant micropropagation.- Chapter 11. Microponic culture system in the propagation of some plants.- Chapter 12. The application of thin cell layers culture technique in plant regeneration and micropropagation - latest achievements.- Chapter 13. In vitro flowering of T. fournieri.- Chapter 14. The use of silver nanoparticles as a disinfectant and media additive in plant micropropagation.- Chapter 15. Enhanced growth and overcoming abnormal phenomena in micropropagation by nanoparticles.- Chapter 16. A protocol of shoot regeneratin and polyploid plantlet production in P. villosum.- Chapter 17. In vitro growth and development of plants under stimulated microgravity condition.- Chapter 18. Wireless light-emitting diode system for micropropagating of Chrysanthemum and Strawberry.