PCR: Clinical Diagnostics and Research

Designed as a laboratory manual, this book provides details on PCR procedures and a discussion of contemporary opinion on the use of this diagnostic method. It examines the most important PCR equipment and the pros and cons of the individual components necessary to perform PCR reactions.

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This laboratory manual provides information about PCR procedures, combined with a discussion of contemporary opinions on this multifaceted method and its applications. Even experienced laboratories will welcome the clear presentation of demanding procedures such as the exact quanitification of PCR products or the in-situ PCR. Protocols that have been tried and tested provide swift support for troubleshooting all sorts of procedural problems. Indeed, many examples of the problem areas found in diagnostically oriented PCR are fully described. Furthermore, the most important lab PCR equipment as well as the pros and cons of single individual reaction components which are necessary for performing PCR reactions are discussed. The book is clearly organized to give an introduction to each technique, brief background information, step-by-step instructions for carrying out the procedure and additional comments.