Metabolic Reprogramming
Springer-Verlag New York Inc.
978-1-0716-3246-8 (ISBN)
Authoritative and cutting-edge, Metabolic Reprogramming: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.
In Vivo Tissue Lipid Uptake in Antisense Oligonucleotides (ASO) Treated Mice.- In Vivo Hepatic Triglyceride Secretion Rate in Antisense Oligonucleotides (ASO) Treated Mice.- Measurement of Fatty Acid Oxidation by High Resolution Respirometry: Special Considerations for Analysis of Skeletal and Cardiac Muscle and Adipose Tissue.- In Vivo Imaging of Bone Marrow Long Chain Fatty Acid Uptake.- Quantification and Tracing of Stable Isotope into Cysteine and Glutathione.- Targeted Quantification of Amino Acids by Dansylation.- Isolation of Mitochondria from Mouse Tissues for Functional Analysis.-Methods for Monitoring Mitochondrial Biogenesis and Turnover in Cultured Hepatocytes and Mouse Liver Using MitoTimer Reporter Assay.- Quantification of Intracellular ATP Content in Ex-Vivo GC B Cells.-UHPLC-HRMS-based Analysis of S-Hydroxymethyl-Glutathione, GSH and GSSG in Human Cells.- Quantitation of glutathione and oxidized glutathione ratios from biological matrices using LC-MS/MS.- Real-Time Monitoring of Hydrogen Peroxide Levels in Yeast and Mammalian Cells.- Tracer-Based Metabolic Analysis by NMR in Intact Perfused Human Liver Tissue.- 13C isotope labeling and mass spectrometric isotope enrichment analysis in acute brain slices.- Metabolite analyses using Nuclear Magnetic Resonance (NMR) spectroscopy in plasma of patients with prostate cancer.- Screening Kinase-Dependent Phosphorylation of Key Metabolic Reprogramming Regulators.- Measuring the Oxidation State and Enzymatic Activity of Glyceraldehyde Phosphate Dehydrogenase (GAPDH).- Quantitation of Glutamine Synthetase-1 Activity in Drosophila Melanogaster.- Fixed Cell Immunofluorescence for Quantification of Hypoxia-Induced Changes in Histone Methylation.- Metabolic Reprogramming During B Cell Differentiation.- An Integrated Methodology to Quantify the Glycolytic Stress in Plasma Cell Myeloma in Response to Cytotoxic Drugs.- Spatial Analysis of Nucleotide Metabolism: From CRISPR Knock Out Cancer Cells to MALDI Imaging of Tumors.- Assessment of Metabolic Pathways and Parameters in Extracellular Matrix-Detached Cells.- Metabolic Networks: Weighted Gene Correlation Network Analysis.
Erscheinungsdatum | 06.06.2023 |
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Reihe/Serie | Methods in Molecular Biology |
Zusatzinfo | 1 Illustrations, black and white; XIV, 330 p. 1 illus. |
Verlagsort | New York, NY |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie |
Schlagworte | GEO DataSets • Metabolic reprogramming • Mitobiogenesis • PET/CT • rate-limiting enzymes |
ISBN-10 | 1-0716-3246-9 / 1071632469 |
ISBN-13 | 978-1-0716-3246-8 / 9781071632468 |
Zustand | Neuware |
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