Crystallography of Protein Dynamics

Nozomi Ando, PhD, is an Associate Professor of Chemistry and Chemical Biology at the Cornell University. Prof. Ando was born in Denver, CO and grew up in the suburbs of Boston, MA. She received her BS from MIT where she was a physics major and music performance minor. She was drawn to the spaceship-like feel of the Cornell High Energy Synchrotron Source (CHESS) and went to Cornell University for her PhD in physics. As a graduate student in Sol Gruner’s lab, she made her own diamond cells for high-pressure X-ray scattering studies. She then went on to work as a postdoctoral fellow in Cathy Drennan’s lab at MIT, where she developed a fascination with metalloenzymes. In 2014, she was appointed Assistant Professor of Chemistry at Princeton University and started a research program that combines X-ray physics and structural enzymology. In 2018, the Ando lab moved to Cornell University when she joined the faculty in Chemistry & Chemical Biology.

1. Introduction to diffuse scattering and data collection
Nozomi Ando, Neti Bhatt, Haoyue Wang, Xiaokun Pei and Steve Meisburger
2. Processing macromolecular diffuse scattering data
Nozomi Ando and Steve Meisburger
3. Molecular dynamics (MD) simulations of biological diffuse scattering
David Wych and Michael Wall
4. Analysis of MD simulations of protein crystals
David Wych and Michael Wall
5. MD Simulations of macromolecular crystals: Implications for the analysis of diffuse scattering
David Case
6. Modeling experimental diffuse scattering with non-MD methods
Thomas J. Lane, Ariana Peck and Fred Poitevin
7. Interpreting Macromolecular Diffraction Through Simulation
Nicholas K. Sauter and Iris D. Young
8. How to refine multiconformer and ensemble models into high resolution multitemperature diffraction data
James Fraser, Siyuan Du and Daniel Herschlag
9. Combining temperature perturbations with X-ray crystallography to study dynamic macromolecules
Michael Thompson
10. Room temperature crystallography and X-ray spectroscopy of metalloenzymes
Junko Yano
11. Pressure perturbation crystallography
Eric Girard, Nathalie Colloc’h and Anne-Claire Dhaussy