Steroid Biochemistry

Dr. Trevor Penning, the Thelma Brown and Henry Charles Molinoff Professor of Pharmacology, is a professor of Systems Pharmacology and Translational Therapeutics at the University of Pennsylvania and has been the Director of the Center of Excellence in Environmental Toxicology (CEET) since its founding in 2006. He formulated the strategic vision of the CEET from its inception and built the environmental health science identity of the CEET at the University, regional, and national level. As Director of the CEET, Dr. Penning has focused the center’s research around the connections between human disease and environmental exposures and emphasized the importance of using this research to mitigate health impacts on vulnerable communities both locally and globally. Throughout his time as Director, Dr. Penning has focused his research on the connections between human health and anthropogenic pollution. Air pollution is responsible for more than 200,000 deaths globally each year and has been identified as a known human carcinogen by the World Health Organization.

Section I. Cytochrome P450 Enzymes 1. Steroidogenic P450s (CYP11A1, 17A1, 21A2, 11B1, and 11B5) Emily Scott 2. Steroid 17alpha-hydroxylase/17,20-lyase (Cytochrome P450 17A1) F. Peter Guengerich, Yasuhiro Tateishi, Kevin D. McCarty and Lu Liu

Section 2. Enzymes of Estrogen Biosynthesis 3. Aromatase and Steroid Sulfatase Debashi Ghosh 4. Estrogenic 17b-Hydroxysteroid Dehydrogenase

Section 3. Hydroxysteroid Dehydrogenases (HSD) 5. 3a-Hydroxyssteroid Dehydrogenase(s) 6. Approaches to Measuring 3b-Hydroxysteroid Dehydrogenase Type 1 Nima Sharifi 7. 3b-Hydroxysteroid Dehydrogenase Type 2 8. 11b-Hydroxysteroids Dehydrogenase Type 1 Alex Odermatt 9. 11b-Hydroxysteroids Dehydrogenase Type 2 Alex Odermatt 10. 17b-Hydroxysteroid Dehydrogenases Tea Lanisnik Rizner

Section 4. Steroid Reductases 11. Approaches for evolutionary, biochemical, and structural analysis of bacterial steroid 5a-reductases Ren Ruobing 12. Mammalian Steroid 5a-Reductase Richard J. Auchus 13. Human Steroid 5b-Reductase (AKR1D1) Trevor Martin Penning 14. Human Aldo-Keto Reductases (AKR1C1-AKR1C4)-Cloning & Expression of Recombinant Enzymes Trevor Martin Penning and Andrea Detlefsen

Section 5. Steroid Conjugating Enzymes 15. Steroid sulfotransferases-X SULT2A1, 2B1b and 1E1 Charles Napoleon Falany 16. Steroid UGT-Glucuronsyl Transfereases

Section 6. Steroid Analytical Chemistry 17. Mass spectrometry of Glucocorticiids 18. Mass spectrometry of Progestins 19. LC-MS Quantitation of Hydroxy- and Keto-Androgens Clementina Mesaros, Andrea Detlefsen and Ryan Paulukinas 20. Quantification of 11-oxygenated androgens by RP-UV-HPLC post 2,4-dinitrophenylhydrazine derivatization Ryan Paulukinas 21. 11-Oxygenated Androgens Amanda Swart 22. LC-MS Quantitation of Estrogens Clementina Mesaros