Proteomics Mass Spectrometry Methods

Dr. Meleady received her degree in Biotechnology from the Dublin City University and PhD from the National Cell and Tissue Culture Centre. She is currently Senior Research Scientist, Programme Leader, Proteomics Core Facility, and Associate Director National Institute for Cell Biology.

Section I. Introduction
1. Book introduction
2. MS Proteomic Overview

Section II. Sample Preparation
3. Sample preparation from mammalian cell lines
4. Sample preparation for proteomics and MS from clinical tissue
5. Sample preparation for proteomics and MS from biological fluids, e.g. serum/plasma from patient blood
6. Sample preparation for proteomics and MS from bacterial and fungal samples
7. Sample preparation for proteomics and MS from urine samples including exosome isolation
8. Sample preparation for proteomics and MS from animal samples
9. Subcellular fractionation methods for proteomic analysis

Section III. Protein Digestion
10. In gel digestion for mass spectrometry
11. In solution digestion for mass spectrometry

Section IV. MS Proteomic Analysis Protocols
12. Protocols for Label-free LC-MS/MS proteomic analysis
13. Protocols for TMT labelled LC-MS/MS analysis
14. Phosphorylation enrichment for phosphoproteomic analysis
15. Ubiquitination enrichment for ubiquitinome PTM analysis
16. Glycosylation enrichment and analysis
17. LC-MS for protein identification and PTM analysis (e.g. methylation)