Cilia: From Mechanisms to Disease–Part B

Jose Manuel Bravo-San Pedro is currently a researcher at the Department of Physiology of the Complutense University of Madrid thanks to a Ramon y Cajal contract grant. He got his Ph.D. in biochemistry, cellular biology and genetics from the University of Extremadura (Caceres, Spain) in 2011, and he did a post-doctoral stage in the laboratory of Prof. Guido Kroemer. His main research interests have always been linked to autophagy, addressing this cellular process associated with neurodegenerative diseases or cancer and recently obesity and specifically related to problems in the correct functioning of the cilium. He is co-inventor of two patents and co-author of 110 publications indexed in PubMed in prestigious international journals, with h-index 45 and 23768 cites (Dec 2022). Lorenzo Galluzzi is Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College, Honorary Assistant Professor Adjunct with the Department of Dermatology of the Yale School of Medicine, Honorary Associate Professor with the Faculty of Medicine of the University of Paris, and Faculty Member with the Graduate School of Biomedical Sciences and Biotechnology of the University of Ferrara, the Graduate School of Pharmacological Sciences of the University of Padova, and the Graduate School of Network Oncology and Precision Medicine of the University of Rome “La Sapienza”. Moreover, he is Associate Director of the European Academy for Tumor Immunology and Founding Member of the European Research Institute for Integrated Cellular Pathology. Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. He has published over 450 articles in international peer-reviewed journals and is the Editor-in-Chief of four journals: OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, Methods in Cell biology, and Molecular and Cellular Oncology (which he co-founded in 2013). Additionally, he serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease, Pharmacological Research and iScience.

1. The challenge of dissecting gene function in model organisms: tools to characterize genetic mutants and assess transcriptional adaptation in zebrafish
Magdalena Cardenas Rodriguez and Iain Drummond
2. Human LUHMES and NES cells as models for studying primary cilia in neurons
Peter Swoboda
3. Fixation methods and immunolabeling for cilia proteins in ciliary and extraciliary locations
Russell J. Ferland
4. Single-molecule imaging in the primary cilium
Lucien E. Weiss
5. Methods to analyze primary cilia in mouse cardiac lesion model
Alfredo Criollo Sr., Daniel Peña-Oyarzun, Rodrigo Troncoso, Joseph A. Hill, Sergio Lavandero and Mauricio Budini Sr.
6. Methods to study motile ciliated cell types in a zebrafish brain mode
Nathalie Jurisch-Yaksi
7. Clinical and Molecular diagnosis in BBS (Bardet-Biedl syndrome)
Diana Valverde
8. Modelling ciliopathies in patient-derived primary cells
Markus Schueler and Kai-Uwe Eckardt
9. Analysis of motility and mucociliary function of tracheal epithelial cilia
Koji Ikegami
10. High-speed Super-resolution SPEED Microscopy to study Live Primary Cilium
Weidong Yang
11. Method for siRNA delivery in retina explants
Florent Poulhès
12. Methods to study primary cilia and autophagy in the brain
Olatz Pampliega
13. Multi-color live-cell fluorescence imaging of primary ciliary membrane assembly dynamics
Christopher Westlake