Craniofacial Development

1. Using Caenorhabditis elegans as a model for mechanistic insights of craniofacial development.- Wholemount In-situ Hybridisation (WISH) in Zebrafish Embryos to Analyse Craniofacial Developmen.- Elucidationof Gene Expression Patterns in The Craniofacial Tissues Of Mouse Embryos By Whole-MountIn Situ Hybridization.- Visualizationof the Cartilage and Bone Elements in The Craniofacial Structures by Alcian blue and Alizarin Red Staining.- Methodology for Free-Floating Organ Culture of Mid-Gestation Maxillary Primordial Tissue.- Methods of Palate Culture in Later Palatogenesis: Elevation, Horizontal Outgrowth, and Fusion.- Single cell RNA-seq: Cell Isolation and Data Analysis.- Generating Zebrafish RNA-less Mutant Alleles by Deleting Gene Promoters with CRISPR/Cas9.- Craniofacial Phenomics: Three-Dimensional Assessment of the Size and Shape of Cranial and Dentofacial Structures.- Micro-CT-based Bone Microarchitecture Analysis of the Murine Skull.- Characterization of mammalian in vivo enhancers using mouse transgenesis and CRISPR genome editing.- Histological Techniques for Sectioning Bones of the Vertebrate Craniofacial Skeleton.- Engineering Epithelial-Mesenchymal Microtissues to Study Cell-Cell Interactions in Development.- Ex Vivo Culture of Human Cranial Suture Cells.- Scaffolds for Use in Craniofacial Bone Regeneration.- Mandible Explant Assay for The Analysis of Meckel’s Cartilage Development.- High Resolution Histology for Craniofacial Studies On Zebrafish and Other Teleost Models.- Live Imaging the Dynamics Of Mammalian Neural Crest Cell Migration.- Salivary Gland Development In Culture.- Antenatal Ultrasound Imaging for Analysis of Human Craniosynostosis.