Drosophila Oogenesis
Springer-Verlag New York Inc.
978-1-0716-2969-7 (ISBN)
Authoritative and practical, Drosophila Oogenesis: Methods and Protocols serves as an ideal guide for both new and expert researchers working with this robust and versatile model organism.
The Vast Utility of Drosophila Oogenesis.- Visualization and Quantification of Drosophila Larval Ovaries.- Dissection, Fixation, and Standard Staining of Adult Drosophila Ovaries.- Utilizing the FLP-Out System for Clonal RNAi Analysis in the Adult Drosophila Ovary.- Analysis of Physiological Control of Adult Drosophila Oogenesis by Inter-Organ Communication.- Immunohistochemical Analysis of Nuclear Lamina Structures in the Drosophila Ovary Using CRISPR-Tagged Genes.- Visualizing Fusome Morphology via Tubulin Immunofluorescence in Drosophila Ovarian Germ Cells.- Tracking Follicle Cell Development.- Optimized Fixation and Phalloidin Staining of Basally Localized F-Actin Networks in Collectively Migrating Follicle Cells.- Quantitative Image Analysis of Dynamic Cell Behaviors during Border Cell Migration.- Live Imaging of Nurse Cell Behavior in Late Stages of Drosophila Oogenesis.- Visualizing Lipid Droplets in Drosophila Oogenesis.- Assessing Ovulation in Drosophila melanogaster.- A Low-Tech Flow Chamber for Live Imaging of Drosophila Egg Chambers during Drug Treatments.- Detection and Assessment of Wolbachia pipientis Infection.- Measuring Transposable Element Activity in Adult Drosophila Ovaries.- Preparation of Drosophila Ovarioles for Single-Cell RNA Sequencing.- Chromatin Immunoprecipitation Experiments from Drosophila Ovaries.- Detection of Actin in Nuclear Protein Fraction Isolated from Adult Drosophila Ovary.- STAMP: Spatio-Temporal Association Mapping of Proteins.- Using Drosophila Oogenesis in the Classroom to Increase Student Participation in Biomedical Research.- Gaining Wings to FLY: Using Drosophila Oogenesis as an Entry Point for Citizen Scientists in Laboratory Research.
Erscheinungsdatum | 02.02.2023 |
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Reihe/Serie | Methods in Molecular Biology ; 2626 |
Zusatzinfo | 82 Illustrations, color; 10 Illustrations, black and white; XIII, 447 p. 92 illus., 82 illus. in color. With online files/update. |
Verlagsort | New York, NY |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Allgemeines / Lexika |
Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie | |
Naturwissenschaften ► Biologie ► Zoologie | |
ISBN-10 | 1-0716-2969-7 / 1071629697 |
ISBN-13 | 978-1-0716-2969-7 / 9781071629697 |
Zustand | Neuware |
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