Optical Microscopy

Dr. Lemasters' research is focused on developing and applying new techniques of laser scanning confocal and multiphoton microscopy to characterize the physiology of single living cells, including the assessment of ion homeostasis, mitochondrial function, electrical potentials, oxygen and nitrogen free radical formation, membrane permeability and other biochemical parameters during the pathogenesis of lethal cell injury.

M. Peonie, New Fluorescent Probes of Cell Function. J.P.Y. Kao and S.R. Adams, Photosensitive Caged Compounds: Design, Properties, And Biological Applications. G.R. Bright, Fluorescence Ratio Imaging: Errors and Artifacts. R.J. Bookman and F.T. Horrigan, Sampling Characteristics of Ccd Video Cameras. C.S. Chew and M. Ljungstrom, Measurement and Manipulation of Oscillations in Cytoplasmic Calcium. S.J. Morris, Simultaneous Multiple Detection of Fluorescent Molecules: Rapid Kinetic Imagin of Calcium and Ph in Living Cells. E. Niggli, R.W. Hadley, M.S. Kirby, And W.J. Lederer, Real-Time Fluorescence Imaging, Photolysis of Caged Compounds, And Whole-Cell Patch Clamping. J.K. Foskett, Simultaneous Differential Interference Contrase and Quantitative Low-Light Fluorescence Video Imaging of Cell Function. W. Rodgers and M. Glaser, Fluorescence Microscopic Imaging of Membrane Domains. M.P. Sheetz and E.L. Elson, Measurement of Membrance Glycoprotein Movement By Single Particle Tracking. L.K. Tamm, Total Internal Reflectance Fluorescence Microscopy. J.J. Lemasters, E. Chacon, G. Zahrebelski, J.M. Reece, And A.-L. Nieminen, Laser Scanning Confocal Microscopy of Living Cells. R.S. Mccuskey, Intravital Microscopy. E. Gratton and M.J. Vandeven, Time-Resolved Fluorescence Lifetime Imaging. S.J. Lockett, M. Siadat-Pajouh, K. Jacobson, And B. Herman, Automated Fluorescence Image Cytometry as Applied to the Diagnosis and Understanding of Cervical Cancer. Chapter References. Index.4