Emerging Model Organisms

Establish a fishroom for African turquoise killifish Nothobranchius furzeri.- Gene editing and transgenic reporter assays in African killifish.- Spinal cord injury in African killifish.- Whole brain clearing and immunolabelling in the African killifish N. furzeri.- Studying Mating Behaviors in Nothobranchius furzeri.- A chromatin immunoprecipitation (ChIP) protocol for African turquoise killifish.- EdU labeling of proliferating cells in the African killifish Nothobranchius furzeri.- Immunofluorescence staining on cryosections with high autofluorescence in African killifish.- Rapid visualization of gene expression using chromogenic RNA in situ hybridization in African killifish.- Profiling gene expression in African turquoise killifish Nothobranchius furzeri embryos with RNA fluorescence in situ hybridization chain reaction (HCR).- Cell dissociation of tissues for single-cell analysis in African killifish.- Cryopreservation of the Mexican Tetra: Astyanax mexicanus.- Whole-mount multicolor fluorescent labeling by in situ hybridization in Astyanax mexicanus embryos and larvae.- Application of CRISPR-Cas9 for functional analysis in A. mexicanus.- Immunohistochemistry and in vivo neural imaging in A. mexicanus.- Sleep behavior analysis in Astyanax mexicanus.- A new method to analyze non-visual based social-like interactions in asocial cavefish.- A generalist’s guide to quantifying swimming behavior within traditional and non-traditional teleost models.- Recording acoustic behaviour in Astyanax mexicanus fish:acquisition, decryption, interpretation.- Generation of mini-brains from hiPSCs.- Generating Human Pluripotent Stem Cell-Derived AssemBloids™ to Model Interneuron Migration and Immune Cell Interactions.- Multiplexed quantitative proteomics analysis of developing human brain organoids.