Signal Transduction Immunohistochemistry
Springer-Verlag New York Inc.
978-1-0716-2810-2 (ISBN)
Authoritative and up-to-date, Signal Transduction Immunohistochemistry: Methods and Protocols, Third Edition aims to arm novices and experts with vital protocols they can use either as-is or tailor them for specific experimental needs.
Top 5 Considerations When Choosing an Antibody.- Validating Antibody Specificities for Immunohistochemistry by Protein Blotting.- Recent Advances in Chromogens for Immunohistochemistry.- Overview of Imaging Techniques in Immunohistochemistry.- Visualizing Activated Myofibroblasts Resulting from Differentiation of Mesenchymal Stem Cells.- Clinical Applications of Flow Cytometry in Cancer Immunotherapies: From Diagnosis to Treatments.- Labeling of Phospho-Specific Antibodies with oYo-Link® Epitope Tags for Multiplex Immunostaining.- Multiplex Immunochromogenic Tissue Staining Employing Primary Antibodies from the Same Species.- 3D Imaging for Cleared Tissues and Thicker Samples on Confocal and Light-Sheet Microscopes.- Optical Tissue Clearing Enables Three-Dimensional Morphometry in Experimental Nerve Regeneration Research.- Protocol and Software for Automated Detection of Lysosome Active “Runs” and “Flights” with Wavelet Transform Approach.- Phenotyping of M1 and M2a Macrophages and Differential Expression of ACE-2 on Monocytes by Flow Cytometry: Impact of Cell Culture Conditions and Sample Processing.- In Situ Hybridization (ISH) Combined with Immunocytochemistry (ICC) Co-Detection of Phosphorylated EGFR in A431 Cultured Cells.- In Situ Hybridization (ISH) Combined with Immunohistochemistry (IHC) for Co-Detection of EGFR RNA and Phosphorylated EGFR Protein in Lung Cancer Tissue.- Multiplex Spatial Protein Detection by Combining Immunofluorescence with Immunohistochemistry.- Transcription Factor-Mediated Differentiation of Motor Neurons from Human Pluripotent Stem Cells.- Immunohistochemical Techniques for Phosphoproteins.- Visualizing Cofilin-Actin Filaments by Immunofluorescence and CryoEM: Essential Steps for Observing Cofilactin in Cells.- Cyclic Multiplex Fluorescent Immunohistochemistry Protocol to Phenotype Glial Cells in Human Formalin-Fixed Paraffin-Embedded Brain Sections.- Spatially Characterizing the Immune Contexture in Mouse Tissue Using Multiplex Immunohistochemistry.- Highly Multiplexed Immuno-Fluorescence Imaging for Quantitative Spatial Analysis in Tissue Samples with ChipCytometryTM.
Erscheinungsdatum | 17.12.2022 |
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Reihe/Serie | Methods in Molecular Biology ; 2593 |
Zusatzinfo | 67 Illustrations, color; 17 Illustrations, black and white; XI, 328 p. 84 illus., 67 illus. in color. With online files/update. |
Verlagsort | New York, NY |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Allgemeines / Lexika |
Naturwissenschaften ► Chemie | |
Schlagworte | antibodies • Biotechnology • drug discovery • flow cytometry • Multiplex IHC • Signal transduction targets |
ISBN-10 | 1-0716-2810-0 / 1071628100 |
ISBN-13 | 978-1-0716-2810-2 / 9781071628102 |
Zustand | Neuware |
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