Receptor and Ion Channel Detection in the Brain

Production of High-Quality Antibodies for the Study of Receptors and Ion Channels.- Co-Immunoprecipitation from Brain.- Sub-Synaptic Membrane Fractionation.- Quantitative Analysis of Cell Surface Expressed, Intracellular, and Internalized Neurotransmitter Receptor Populations in Brain Slices Using Biotinylation.- Single Nanoparticle Tracking of Surface Ion Channels and Receptors in Brain Cells.- Radioligand Binding Detection of Receptors in Brain Membranes.- Recombinant Alphavirus-Mediated Expression of Ion Channels and Receptors in the Brain.- Time Resolved-Fluorescence Resonance Energy Transfer using Fluorescent Ligands to Study Native G Protein-Coupled Receptor Heteromerization in Brain.- Study of GPCR Homo and Heteroreceptor Complexes in Specific Neuronal Cell Populations using the In Situ Proximity Ligation Assay.- Amplified Luminescent Proximity Homogeneous Assay (Alpha)-Based Technique to Detect GPCR Oligomers in Human Post-Mortem Brain.- Fluorescent In Situ Hybridization for Sensitive and Specific Labeling.- Autoradiographic Visualization of G Protein-Coupled Receptors in Brain.- Localization of Neurotransmitter Receptor and Ion Channel Proteins in Unfixed Brains using In Situ Immunoblotting.- Immunohistochemistry for Ion Channels and their Interacting Molecules: Tips for Improving Antibody Accessibility.- Localization of GFP-Tagged Proteins at the Electron Microscope.- Tyramide Signal Amplificación for Immunoelectron Microscopy.- Pre-Embedding Methods for the Localization of Receptors and Ion Channels.- Post-Embedding Immunohistochemistry in the Localization of Receptors and Ion Channels.- High-Resolution Localization and Quantitation of Membrane Proteins by SDS-Digested Freeze-Fracture Replica Labeling (SDS-FRL).- Pre-Embedding Immunostaining of Brain Tissue and Three-Dimensional Imaging with FIB-SEM.- Application of Virus Vectors for Anterograde Tract-Tracing and Single-Neuron Labeling Studies.- Efficient Labeling of Neurons and Identification of Postsynaptic Sites Using Adeno-Associated Virus Vector.- Analysis of Synaptic Connections at the Electron Microscopic Level using Sindbis Virus Vectors.- Morphological and Neurochemical Characterization of Electrophysiologically Identified Cells.- Using Electrophysiology to Study Synaptic and Extrasynaptic Ionotropic Receptors in Hippocampal Neurons.- Biophysical Methods to Analyze Direct G-Protein Regulation of Neuronal Voltage-Gated Calcium Channels.- Electrophysiological Recordings in Behaving Animals.- Voltammetry in Behaving Animals.- In Vivo Brain Microdialysis of Monoamines.- Optical Control of Brain Receptors using Photoactive Drug in Behaving Animals.- Dynamic Recording of Membrane Potential from Hippocampal Neurons by Using a Fluorescence Resonance Energy Transfer-Based Voltage Biosensor.- Monitoring GPCR-Mediated cAMP Accumulation in Rat Striatal Synaptosomes.- GPCR-Mediated MAPK/ERK Cascade Activation in Mouse Striatal Slices.