Fe-S Proteins
Springer-Verlag New York Inc.
978-1-0716-1607-9 (ISBN)
Authoritative and cutting-edge, Fe-S Proteins: Methods and Protocols aims to be a useful practical guide to researchers to help further their study in this field.
Molecular biology and genetic tools to investigate functional redundancy among Fe-S cluster carriers in E. coli.- Study Fe-S cluster proteins in Methanococcus maripaludis, a model archaeal organism.- Genetic approaches to uncover gene products involved in iron-sulfur protein maturation: High throughput genomic screening using transposon-sequencing.- Methods for heterologous overproduction of Fe-S proteins.- Enzymatic and Chemical in vitro Reconstitution of Iron-Sulfur Cluster Proteins.- Expression, isolation, and characterization of vanadium nitrogenase from Azotobacter vinelandii.- Ni-NTA affinity chromatography to characterize protein-protein interactions during Fe-S cluster biogenesis.- tRNA modifications as a readout of S and Fe-S metabolism.- Methods to unravel the roles of ATPases in Fe–S cluster biosynthesis.- Methods to investigate the kinetic profile of cysteine desulfurases.- A fast and ratiometric method for quantification of cysteine-bound persulfides based on alkylation and gel-shift assays.- Circular dichroism to probe the synthesis, transfer, and stability of Fe-S clusters.- Native mass spectrometry of iron-sulfur proteins.- Characterization of Paramagnetic Iron-Sulfur Clusters using Electron Paramagnetic Resonance Spectroscopy.- Characterization of Fe-S clusters in proteins by Mӧssbauer Spectroscopy.- Biochemical approaches to probe the role of the auxiliary iron-sulfur cluster of lipoyl synthase from Mycobacterium tuberculosis.- Methods to Screen for Radical SAM Enzyme Crystallization Conditions.
Erscheinungsdatum | 28.07.2022 |
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Reihe/Serie | Methods in Molecular Biology ; 2353 |
Zusatzinfo | 64 Illustrations, color; 23 Illustrations, black and white; XI, 351 p. 87 illus., 64 illus. in color. |
Verlagsort | New York, NY |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Biochemie |
Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie | |
Schlagworte | Archaeal enzymes • dinitrogen reduction • eukaryotic systems • metallocofactors • nitrogenase enzymes |
ISBN-10 | 1-0716-1607-2 / 1071616072 |
ISBN-13 | 978-1-0716-1607-9 / 9781071616079 |
Zustand | Neuware |
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