Reproductive Technologies and Biobanking for the Conservation of Amphibians

Andy J. Kouba is the Wildlife, Fisheries and Aquaculture Department Head at Mississippi State University, Executive Director of the Center for Human-Wildlife Interactions, and Co-Director of the National Amphibian Genome Bank. Aimee J. Silla is an Associate Research Fellow at the University of Wollongong and co-head of the Evolution and Assisted Reproduction Laboratory. Her integrative research advancing reproductive technologies is promoting new directions for threatened species recovery. Harold Heatwole has four doctorates and holds Professorships at North Carolina State University and University of New England. He researches herpetology, biogeography, polar ecosystems, vegetation dynamics, as well as ants, tardigrades, and seabirds. Harold Heatwole was also a co-editor of Volume 11, Part 6 of the Amphibian Biology series, Status of Conservation and Decline of Amphibians: Australia, New Zealand, and Pacific Islands (CSIRO Publishing, 2018).

Dedication

List of contributors

Preface

Volumes in the series Amphibian Biology



1. Integrating reproductive technologies into the conservation toolbox for the recovery of amphibian species

2. Status of global amphibian declines and the prioritisation of species for captive breeding

3. Methods of identifying the sex of amphibians and of conditioning captive brood stock for assisted reproduction



Colour plates



4. Hormonal control of amphibian reproduction

5. Non-invasive monitoring of stress physiology during management and breeding of amphibians in captivity

6. Ultrasonography to assess female reproductive status and inform hormonally induced ovulation

7. Protocols for hormonally induced spermiation, and the cold storage, activation, and assessment of amphibian sperm

8. Genetic management of threatened amphibians: using artificial fertilisation technologies to facilitate genetic rescue and assisted gene flow

9. Cryopreservation of amphibian genomes: targeting the Holy Grail, cryopreservation of maternal-haploid and embryonic-diploid genomes

10. Culturing and biobanking of amphibian cell lines for conservation applications

11. Linking in situ and ex situ populations of threatened amphibian species using genome resource banks

12. Genome resource banks as a tool for amphibian conservation



Appendix

Index