Quantitative Analysis of Cellular Drug Transport, Disposition, and Delivery

Measurement of Transcellular Transport Rates and Intracellular Drug Sequestration in the Presence of an Extracellular Concentration Gradient.- Kinetic Design for Establishing Long Term Stationary Cytosol Concentrations during Drug Transport across P-gp Expressing Confluent Cell Monolayers to Facilitate Measuring Cytosol Concentration, Fitting Drug Molar Partition Coefficients into the Cytosolic Monolayer of the Plasma Membrane and Kinetically Identifying Drug Uptake Transporters.- In Vitro Methodologies to Assess Potential for Transporter-Mediated Drug-Drug Interactions.- Determination of Fraction Unbound and Unbound Partition Coefficient to Estimate Intracellular Free Drug Concentration.- Quantitative Analysis of Intracellular Drug Concentrations in Hepatocytes.- Quantification of Intracellular Drug Aggregates and Precipitates.- Quantitative Phenotypic Analysis of Drug Sequestering Macrophage Subpopulations.- Using an Integrated QSAR Model to Check Whether Small-Molecule Xenobiotics Will Accumulate in Biomembranes, with Particular Reference to Fluorescent Imaging Probes.- Diversity-Oriented Fluorescence Library Approach (DOFLA) for Discovery of Cell-Permeable Probes for Applications in Live Cell Imaging.- Overcoming Cellular and Systemic Barriers to Design the Next Wave of Peptide Therapeutics.- Intracellular Targeting of Cyclotides for Therapeutic Applications.- Cellular Trafficking of Monoclonal and Bispecific Antibodies.- Quantitative Drug Target Imaging Using Paired-Agent Principles.- Quantitative Determination of Intracellular Bond Cleavage.- Development and Application of a Single Cell-Level PK-PD Model for ADCs.- Contribution of Non-Target Cells to the Disposition, Antitumor Activity, and Antigen-Independent Toxicity of Antibody Drug-Conjugates.- Tracking siRNA-Nanocarrier Assembly and Disassembly Using FRET.- Sub-Cellular Drug Depots as Reservoirs for Small Molecule Drugs.