RNA Modifications

RNA Post-Transcriptional Modification Mapping Data Analysis Using RNA Framework.- An Informatics Pipeline for Profiling and Annotating RNA Modifications.- EpiNano: Detection of m6A RNA Modifications Using Oxford Nanopore Direct RNA Sequencing.- Adaptation of Human Ribosomal RNA for Nanopore Sequencing of Canonical and Modified Nucleotides.- AlkAniline-Seq: A Highly Sensitive and Specific Method for Simultaneous Mapping of 7-Methyl-Guanosine (m7G) and 3-Methyl-Cytidine (m3C) in RNAs by High-Throughput Sequencing.- Transcriptome-Wide Detection of Internal N7-Methylguanosine.- miCLIP-MaPseq Identifies Substrates of Radical SAM RNA Methylating Enzyme Using Mechanistic Crosslinking and Mismatch Profiling.- Mapping RNA Modifications Using Photo-Crosslinking-Assisted Modification Sequencing.- Quantitative and Single Nucleotide Resolution Profiling of RNA 5-Methylcytosine.- A Small RNA-Seq Protocol with Less Bias and Improved Capture of 2’-O-Methyl RNAs.- Assessing 2'-O-Methylation of mRNA Using Quantitative PCR.- Relative Quantification of Residue Specific m6A RNA Methylation Using m6A-RT-QPCR.- Monitoring the 5-Methoxycarbonylmethyl-2-Thiouridine (mcm5s2U) Modification Utilizing the Gamma-Toxin Endonuclease.- Analysis of Queuosine tRNA Modifications Using APB Northern Blot Assays.- Detecting ADP-Ribosylation in RNA.- Detecting Internal N7-Methylguanosine mRNA Modifications by Differential Enzymatic Digestion Coupled with Mass Spectrometry Analysis.- A General LC-MS-Based Method for Direct and De Novo Sequencing of RNA Mixtures Containing Both Canonical and Modified Nucleotides.- Quantification of Modified Nucleosides in the Context of NAIL-MS.- A Method to Monitor the Introduction of Post-Transcriptional Modifications in tRNAs with NMR Spectroscopy.- Effects of mRNA Modifications on Translation: An Overview.- Assaying the Molecular Determinants and Kinetics of RNA Pseudouridylation by H/ACA snoRNAs and Stand-Alone Pseudouridine Synthases.- Investigating Pseudouridylation Mechanisms by High-Throughput In Vitro RNA Pseudouridylation and Sequencing.- Targeted RNA m6A Editing Using Engineered CRISPR-Cas9 Conjugates.