RNA Methodologies
Academic Press Inc (Verlag)
978-0-12-249696-7 (ISBN)
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This laboratory guide represents a growing collection of tried, tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA, with lesser emphasis on the characterization of prokaryotic transcripts. Collectively the chapters work together to embellish the RNA story, each presenting clear take-home lessons, liberally incorporating flow charts, tables and graphs to facilitate learning and assist in the planning and implementation phases of a project.RNA Methodologies, 3rd edition includes approximately 30% new material, including chapters on the more recent technologies of RNA interference including: RNAi; Microarrays; Bioinformatics. It also includes new sections on: new and improved RT-PCR techniques; innovative 5’ and 3’ RACE techniques; subtractive PCR methods; methods for improving cDNA synthesis.
Dr. Robert Farrell is a bench-current scientist who has 35 years of experience working with RNA in the study of transcriptional and posttranscriptional regulation of gene expression in a variety of model systems. He is also experienced in animal cell culture methods. Prior to joining the faculty at Penn State University, he operated a biotech education and service firm, winning the 1998 Small Business Contractor of the Year award from the U.S. Department of Agriculture. He is the recipient of campus- and college-wide awards for excellence in teaching, and has extensive experience running RNA and specialized biotechnology hands-on laboratory training programs all over the world. He often serves as a consultant within the pharmaceutical and biotech industries. Dr. Farrell received his Ph.D. and M.S. degrees from The Catholic University of America and his B.S. in Biology from Providence College. Dr. Farrell currently serves as the campus academic officer at Penn State York.
Chapter 1: RNA and the Cellular Biochemistry Revisited
Chapter 2: Transcription and the Organization of Eukaryotic Genes
Chapter 3: Messenger RNA
Chapter 4: Resilient Ribonucleases
Chapter 5: RNA Isolation Strategies
Chapter 6: The Truth About Tissues
Chapter 7: Isolation of Polyadenylated RNA
Chapter 8: Quality Control for RNA Preparations
Chapter 9: Dot Blot Analysis
Chapter 10: Electrophoresis of RNA
Chapter 11: Photodocumentation and Image Analysis
Chapter 12: Northern Analysis
Chapter 13: Nucleic Acid Probe Technology
Chapter 14: Practical Nucleic Acid Hybridization
Chapter 15: Principles of Detection
Chapter 16: Quantification of Specific mRNAs by Nuclease Protection
Chapter 17: Analysis of Nuclear RNA
Chapter 18: cDNA Synthesis
Chapter 19: RT-PCR
Chapter 20: Quantitative PCR Techniques
Chapter 21: Transcript Subtraction Methods
Chapter 22: mRNA Differential Display
Chapter 23: High-Throughput Analysis of Gene Expression
Chapter 24: RNA Interference: Targeted Gene Silencing
Chapter 25: Genomes, Transcriptomes, Proteomes, and Bioinformatics
Chapter 26: An RNA Paradigm
Epilogue: A Few Pearls of Wisdom
Appendix A: Maintaining Complete and Accurate Records
Appendix B: Useful Stock Solutions for the Molecular Biologist
Appendix C: Phenol Preparation
Appendix D: Disposal of Ethidium Bromide and SYBR Green Solutions
Appendix E: DNase I Removal of DNA from an RNA Sample
Appendix F: RNase Incubation to Remove RNA from a DNA Sample
Appendix G: Deionization of Formamide, Formaldehyde, and Glyoxal
Appendix H: Silanizing Centrifuge Tubes and Glassware
Appendix I: Centrifugation as a Mainstream Tool for the Molecular Biologist
Appendix J: Trypsinization Protocol for Anchorage-Dependent Cells
Appendix K: Isolation of High-Molecular-Weight DNA by Salting-Out
Appendix L: RNA Isolation from Plant Tissue
Appendix M: Electrophoresis: Principles, Parameters, and Safety
Appendix N: Polyacrylamide Gel Electrophoresis
Appendix O: Selected Suppliers of Equipment, Reagents, and Services
Appendix P: Useful SI Units
Appendix Q: Common Abbreviations
Appendix R: Trademark Citations
Glossary
Index
| Erscheint lt. Verlag | 6.5.2005 |
|---|---|
| Verlagsort | San Diego |
| Sprache | englisch |
| Maße | 152 x 229 mm |
| Gewicht | 1070 g |
| Themenwelt | Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie |
| ISBN-10 | 0-12-249696-5 / 0122496965 |
| ISBN-13 | 978-0-12-249696-7 / 9780122496967 |
| Zustand | Neuware |
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