Neuronal Cell Culture

General Overview of Neuronal Cell Culture.- Considerations for the use of SH-SY5Y Neuroblastoma Cells in Neurobiology.- Cultured cell line models of neuronal differentiation: NT2, PC12, SK-N-MC.- Murine teratocarcinoma-derived neuronal cultures.- Isolation and propagation of primary human and rodent embryonic neural progenitor cells and cortical neurons.- Mouse Enteric Neuronal Cell Culture.- Preparation of Neural Stem Cells and Progenitors: Neuronal Production and Grafting Applications.- Slice culture modeling of CNS viral infection.- Neurospheres and Glial cell cultures: immunocytochemistry for cell phenotyping.- Transfection of Neuronal Cultures.- Lentiviral Transduction of Neuronal Cells.- Compartmentalized Neuronal Cultures.- Quantitative assessment of neurite outgrowth over growth promoting or inhibitory substrats.- Isolation and Culture of Dorsal Root Ganglia (DRG) from Rodents.- Isolation of Primary Human and Rodent Brain Microvascular Endothelial Cells: Culturing, Characterization, and High-Efficiency Transfection.- Fetal Brain Injury Models of Fetal Alcohol Syndrome: Examination of Neuronal Morphologic Condition Using Sholl Assay.